Isoquinoline Alkaloids and their Binding with DNA: Calorimetry and Thermal Analysis Applications
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Isoquinoline Alkaloids and their Binding with DNA: Calorimetry and Thermal Analysis Applications
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| Creator |
Bhadra, Kakali
Kumar, G Suresh |
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| Subject |
Chemistry
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| Description |
Alkaloids are a group of natural products with unmatched chemical diversity and biological relevance forming
potential quality pools in drug screening. The molecular aspects of their interaction with many cellular macromolecules
like DNA, RNA and proteins are being currently investigated in order to evolve the structure activity relationship. Isoquinolines
constitute an important group of alkaloids. They have extensive utility in cancer therapy and a large volume of
data is now emerging in the literature on their mode, mechanism and specificity of binding to DNA. Thermodynamic
characterization of the binding of these alkaloids to DNA may offer key insights into the molecular aspects that drive
complex formation and these data can provide valuable information about the balance of driving forces. Various thermal
techniques have been conveniently used for this purpose and modern calorimetric instrumentation provides direct and
quick estimation of thermodynamic parameters. Thermal melting studies and calorimetric techniques like isothermal titration
calorimetry and differential scanning calorimetry have further advanced the field by providing authentic, reliable and
sensitive data on various aspects of temperature dependent structural analysis of the interaction. In this review we present
the application of various thermal techniques, viz. isothermal titration calorimetry, differential scanning calorimetry and
optical melting studies in the characterization of drug-DNA interactions with particular emphasis on isoquinoline alkaloid-
DNA interaction.
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| Date |
2010
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1023/1/11_MRMC_2010.pdf
Bhadra, Kakali and Kumar, G Suresh (2010) Isoquinoline Alkaloids and their Binding with DNA: Calorimetry and Thermal Analysis Applications. Mini-Reviews in Medicinal Chemistry, 10 (13). pp. 1235-1247. |
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| Relation |
http://dx.doi.org/
http://www.eprints.iicb.res.in/1023/ |
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