Combination of Ascorbate and a-Tocopherol as a Preventive Therapy against Structural and Functional Defects of Erythrocytes in Visceral Leishmaniasis
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Combination of Ascorbate and a-Tocopherol as a Preventive
Therapy against Structural and Functional Defects of
Erythrocytes in Visceral Leishmaniasis
|
|
| Creator |
Sen, Gargi
Mukhopadhaya, Rupa Ghosal, Jharna Biswas, Tuli |
|
| Subject |
Cell Biology & Physiology
|
|
| Description |
The redox unbalance in erythrocytes has been found to
contribute significantly in the development of anemia in
visceral leishmaniasis (VL). The present study revealed
enhanced production of reactive oxygen species (ROS)
and gradual depletion of a-tocopherol and ascorbate in
the erythrocytes of infected animals. The response of
erythrocytes to chronic treatment with antioxidants was
studied in hamsters during leishmanial infection. Treatment
with a combination of a-tocopherol and ascorbate
proved to be the most effective preventive for the
proteolytic degradation of erythrocyte membrane.
Erythrocytes from infected animals were thermally more
sensitive compared to the control ones. Combination of
both antioxidants was most successful in resisting heat
induced structural defects in the cells. Cross-linking of
membrane proteins subsequent to oxidative damage in
the red cells was accompanied by the formation of high
molecular weight protein band at the top of the resolving
gel in the presence of the cross-linking agent dimethyladepimidate
(DMA). Marked inhibition of cross-linking
was observed with combination of both antioxidants.
Treatment with a-tocopherol and ascorbate together could
withstand osmotic lysis of erythrocytes in the infected
animals very efficiently. Decreased hemoglobin (Hb) level
was successfully replenished and was coupled with
significant increase in the life span of red cells after
treating the animals with both antioxidants. Results
indicate better efficacy of the combination therapy with
a-tocopherol and ascorbate in protecting the erythrocytes
from structural and functional damages during leishmanial
infection.
|
|
| Publisher |
Taylor & Francis
|
|
| Date |
2004
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://www.eprints.iicb.res.in/1024/2/Free_Radic_Research(2004).pdf
Sen, Gargi and Mukhopadhaya, Rupa and Ghosal, Jharna and Biswas, Tuli (2004) Combination of Ascorbate and a-Tocopherol as a Preventive Therapy against Structural and Functional Defects of Erythrocytes in Visceral Leishmaniasis. Free Radical Research, 38 (5). pp. 527-534. ISSN 1071-5762 |
|
| Relation |
http://dx.doi.org/10.1080/10715160410001665253
http://www.eprints.iicb.res.in/1024/ |
|