Altered Erythrocyte Membrane Characteristics During Anemia in Childhood Acute Lymphoblastic Leukemia
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Altered Erythrocyte Membrane Characteristics During Anemia
in Childhood Acute Lymphoblastic Leukemia
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| Creator |
Ghosh, Shyamasree
Bandyopadhyay, Suman Bhattacharya, Dilip K Mandal, Chitra |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Anemia is a prominent feature in children with
acute lymphoblastic leukemia (ALL). To investigate the
erythrocyte features during anemia in these patients, we
studied the altered characters of these cells and oxidative
stress imposed in their serum. This investigation reveals
that erythrocytes from ALL patients show (1) increased
membrane fluidity detected by fluorescence anisotropy
studies, increased osmotic fragility detected by hemolysis
of erythrocytes in different saline concentrations, and
increased hydrophobicity as measured by binding with 8-
anilino-1-naphthalenesulfonic acid, (2) enhanced (~ threefold)
glycosylation and sialylation, monitored by digoxigenin
enzyme assay, and (3) expression of diseasespecific
210, 105, 83, 54, and 28 kDa 9-O-acetyl
sialoglycoconjugates (9-O-AcSGs) demonstrated by Western
blot analysis and fluorescence-activated cell sorter
(FACS) analysis studies using Achatinin-H with specificity
towards 9-O-AcSAá2-6GalNAc as the analytical
probe. (4) In addition, induced oxidative stress was
observed in the sera of these children as indicated by
increased nitric oxide (~ fourfold) and thiobarbituric acid
(TBA) reactive species (twofold) as detected by Griess
reaction and TBA assay, respectively. For all the
experiments, erythrocytes from normal individuals served
as controls. Thus, the altered membrane characteristics
together with their exposure to induced oxidative stress in
serum are found to be a few features restricted to diseased
erythrocytes. Taken together, our results are suggestive of
their interplay in the contribution to the observed anemia
in these patients, which may be exploited for better
management of the disease.
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| Date |
2005
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1079/1/ANNALS_OF_HEMATOLOGY%2C_84(_2)%2C__76%2D84_[96].pdf
Ghosh, Shyamasree and Bandyopadhyay, Suman and Bhattacharya, Dilip K and Mandal, Chitra (2005) Altered Erythrocyte Membrane Characteristics During Anemia in Childhood Acute Lymphoblastic Leukemia. Annals of Hematology, 84. pp. 76-84. |
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| Relation |
http://dx.doi.org/10.1007/s00277-004-0933-0
http://www.eprints.iicb.res.in/1079/ |
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