CSIR Central


Field	Value

Title	High-yielding Plasmid Extraction Method from Acidophilic Heterotrophic Bacteria of the Genus Acidiphilium

Creator	Samarendra K, Singh Banerjee, Pataki C

Subject	Chemistry

Description	Plasmid yield from Acidiphilium strains always had been poor following various standard methods. We adopted some simple modiWcations in the alkaline lysis procedure to get a better yield of plasmid from these bacteria. An approximately 10- to 20-fold increase in the plasmid yield was achieved when harvested Acidiphilium cells were preincubated 16–20 h at pH 6 in nitrogen-free medium. Another independent approach showed that freezing (¡18 to ¡20 °C) of the harvested cells initially and at two subsequent steps in the alkaline lysis procedure of plasmid DNA extraction improved the yield further by 1.5- to 3-fold. The combination of these changes yielded at least 15-to 30-fold more plasmid from various Acidiphilium strains as compared with standard methods.

Date	2006

Type	Article PeerReviewed

Format	application/pdf

Identifier	http://www.eprints.iicb.res.in/1084/1/ANALYTICAL_BIOCHEMISTRY__Volume_356___Issue_2___Pages_229%2D234___[36].pdf Samarendra K, Singh and Banerjee, Pataki C (2006) High-yielding Plasmid Extraction Method from Acidophilic Heterotrophic Bacteria of the Genus Acidiphilium. Analytical Biochemistry, 356 (2). pp. 229-234.

Relation	http://dx.doi.org/10.1016/j.ab.2006.06.016 http://www.eprints.iicb.res.in/1084/