HPTLC Determination of Swertiamarin and Amarogentin in Swertia Species from the Western Himalayas
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
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| Title |
HPTLC Determination of Swertiamarin and Amarogentin in
Swertia Species from the Western Himalayas
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| Creator |
Bhandari, P
Singh , Bikram Gupta , A P Kaul , V K |
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| Subject |
Natural Product Chemistry
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| Description |
A high-performance thin-layer chromatographic (HPTLC) method
has been established for simple and rapid quantification of two secoiridoids, swertiamarin and amarogentin, in two Swertia species. HPTLC of secoiridoids was performed on precoated silica 60 F254 plates with EtOAc–MeOH–H2O, 77 + 8 + 8 (v/v), as mobile phase. Densitometric determination of the secoiridoids was performed at λ = 235 nm in reflectance/absorbance mode. The method was validated
for precision, repeatability, and accuracy. Average recovery
of amarogentin and swertiamarin was 94.5 and 96.5%, respectively, showing that the reproducibility of the method was excellent. The method is simple, precise, specific, sensitive, and accurate and can be used for routine quality control of raw materials and herbal formulations.
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| Publisher |
Research Inst Medicinal Plants , PO BOX 11, H-2011 BUDAKALASZ, Hungary
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| Date |
2006
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/51/1/2006_HPTLC_Determination.pdf
Bhandari, P and Singh , Bikram and Gupta , A P and Kaul , V K (2006) HPTLC Determination of Swertiamarin and Amarogentin in Swertia Species from the Western Himalayas. JPC-Journal of Planar Chromatography-Modern TLC , 19 (109). pp. 212-215. ISSN 0933-4173 |
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| Relation |
http://ihbt.csircentral.net/51/
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