Complete nucleotide sequence analysis of Cymbidium mosaic virus Indian isolate: further evidence for natural recombination among potexviruses
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
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| Title |
Complete nucleotide sequence analysis of Cymbidium mosaic
virus Indian isolate: further evidence for natural recombination among potexviruses
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| Creator |
Sherpa, A R
Hallan, Vipin Pathak, P Zaidi, A A |
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| Subject |
Plant Biotechnology
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| Description |
The complete nucleotide sequence of an Indian strain of Cymbidium mosaic virus (CymMV) was determined and
compared with other potexviruses. Phylogenetic analyses on the basis of RNA-dependent RNA polymerase (RdRp),
triple gene block protein and coat protein (CP) amino acid sequences revealed that CymMV is closely related to the
Narcissus mosaic virus (NMV), Scallion virus X (SVX), Pepino mosaic virus (PepMV) and Potato aucuba mosaic
virus (PAMV). Different sets of primers were used for the amplifi cation of different regions of the genome through
RT-PCR and the amplifi ed genes were cloned in a suitable vector. The full genome of the Indian isolate of CymMV
from Phaius tankervilliae shares 96–97% similarity with isolates reported from other countries. It was found that the
CP gene of CymMV shares a high similarity with each other and other potexviruses. One of the Indian isolates seems
to be a recombinant formed by the intermolecular recombination of two other CymMV isolates. The phylogenetic
analyses, Recombination Detection Program (RDP2) analyses and sequence alignment survey provided evidence for
the occurrence of a recombination between an Indian isolate (AM055720) as the major parent, and a Korean type-2
isolate (AF016914) as the minor parent. Recombination was also observed between a Singapore isolate (U62963) as
the major parent, and a Taiwan CymMV (AY571289) as the minor parent.
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| Publisher |
Indian Academy of Science, C V Raman Avenue, Sadashivanagar, P B #8005, Bangalore 560 080, India
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| Date |
2008
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/80/1/2007_Complete.pdf
Sherpa, A R and Hallan, Vipin and Pathak, P and Zaidi, A A (2008) Complete nucleotide sequence analysis of Cymbidium mosaic virus Indian isolate: further evidence for natural recombination among potexviruses. Journal of Biosource, 34 (4). pp. 663-669. ISSN 0250-5991 |
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| Relation |
http://ihbt.csircentral.net/80/
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