Coat protein sequence shows that Cucumber mosaic virus isolate from geraniums (Pelargonium spp.) belongs to subgroup II.
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
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| Title |
Coat protein sequence shows that Cucumber mosaic virus isolate from geraniums (Pelargonium spp.) belongs to subgroup II.
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| Creator |
Verma, N
Mahinaghara, B K Raja, Ram Zaidi, A A |
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| Subject |
Plant sciences
Plant viruses |
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| Description |
A viral disease was identified on geraniums (Pelargonium spp.) grown in a greenhouse at the Institute of Himalayan
Bioresource Technology (IHBT), Palampur, exhibiting mild mottling and stunting. The causal virus (Cucumber
mosaic virus, CMV) was identified and characterized on the basis of host range, aphid transmission, enzyme linked
immunosorbent assay (ELISA), DNA-RNA hybridization and reverse transcription polymerase chain reaction (RTPCR).
A complete coat protein (CP) gene was amplified using degenerate primers and sequenced. The CP gene
showed nucleotide and amino acid homology up to 97%–98% and 96%–99%, respectively with the sequences of
CMV subgroup II. The CP gene also showed homologies of 75%–97% in nucleotide and 77%–96% in amino acid
with the CMV Indian isolates infecting various crops. On the basis of sequence homology, it was concluded that
CMV-infecting geraniums in India belong to subgroup II.
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| Publisher |
Indian Academy of Science, C V Raman Avenue, Sadashivanagar, P B #8005, Bangalore 560 080, India
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| Date |
2006
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/114/1/2006_Coat_portein.pdf
Verma, N and Mahinaghara, B K and Raja, Ram and Zaidi, A A (2006) Coat protein sequence shows that Cucumber mosaic virus isolate from geraniums (Pelargonium spp.) belongs to subgroup II. Journal of Biosource, 31 (1). pp. 47-54. ISSN 0250-5991 |
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| Relation |
http://ihbt.csircentral.net/114/
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