Light and temperature regulated terpene biosynthesis: hepatoprotective monoterpene picroside accumulation in Picrorhiza kurrooa.
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Light and temperature regulated terpene biosynthesis:
hepatoprotective monoterpene picroside accumulation
in Picrorhiza kurrooa.
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| Creator |
Kawoosa, T
Singh, H Kumar, Amit Sharma, S K Devi, K Dutt, Som Vats, S K Sharma, Madhu Ahuja, Paramvir Singh Kumar, Sanjay |
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| Subject |
Plant sciences
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| Description |
Picrorhiza (Picrorhiza kurrooa) is an endangered
medicinal plant with well-known hepatoprotective activity
attributed to monoterpenoid picrosides. The present article
details on regulatory genes of terpenoid metabolism, 3-
hydroxy-3-methylglutaryl coenzyme A reductase (pkhmgr)
and 1-deoxy-D-xylulose-5-phosphate synthase (pkdxs) from
picrorhiza. Since no molecular information was available,
these genes were cloned to full-length by degenerate
primers and rapid amplification of cDNA ends, followed
by cloning of the upstream sequences that showed the
presence of core sequences for light and temperature
responsiveness. Electrophoretic mobility shift assay confirmed
binding of protein to these motifs. Expression of
pkhmgr and pkdxs was up-regulated at 15°C as compared to
at 25°C as well as under light as compared to dark
conditions. Picrosides content exhibited the trend similar
to gene expression. To rule out the possible limitation of
carbon pool under dark condition, plantlets of picrorhiza
were raised in vitro in Murashige and Skoog medium
supplemented with 3% sucrose. Results showed similar upregulation
of both the genes and the higher picrosides
content in in vitro raised plantlets in the presence of light.
Data suggested the important roles played by light and
temperature in regulating pkhmgr and pkdxs, and the
picrosides level in picrorhiza.
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| Publisher |
Springer Heidelberg , Tiergartenstrasse 17, D-69121 Heidelberg, Germany
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| Date |
2010
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/133/1/2010_Light_and_temperature.pdf
Kawoosa, T and Singh, H and Kumar, Amit and Sharma, S K and Devi, K and Dutt, Som and Vats, S K and Sharma, Madhu and Ahuja, Paramvir Singh and Kumar, Sanjay (2010) Light and temperature regulated terpene biosynthesis: hepatoprotective monoterpene picroside accumulation in Picrorhiza kurrooa. Functional and Integrative Genomics, 10 (3). pp. 393-404. ISSN 1438-793X |
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| Relation |
http://ihbt.csircentral.net/133/
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