Reverse transcription polymerase chain reaction-based detection of Arabis mosaic virus and Strawberry latent ringspot virus in vector nematodes.
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Reverse transcription polymerase chain reaction-based detection of Arabis mosaic virus and Strawberry
latent ringspot virus in vector nematodes.
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| Creator |
Kulshrestha, S
Hallan, Vipin Raikhy, G Adekunle, O K Verma, N Haq, Q M R Zaidi, A A |
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| Subject |
Plant sciences
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| Description |
In a survey of rose and lily plantations in experimental
fields of the Institute of Himalayan Bioresource Technology,
Palampur, a number of symptoms characteristic
of nepoviruses were observed. Symptomatic rose
and lily leaves were tested for the presence of nepoviruses.
Strawberry latent ringspot virus (SLRSV) and
Arabis mosaic virus (ArMV) were detected by ELISA
and RT–PCR using virus-specific antibodies and primers
respectively, in rose cultivars Raktgandha, Landora
and Sonia. Only SLRSV was detected in lily cultivars
Galeili, Star Gazer Max and White Merostar. To ascertain
the presence of viruliferous nematodes, soil samples
were collected from around the roots of rose and
lily cultivars. Xiphinema diversicaudatum and Longidorous
macrosoma isolated from soil were placed separately
into 5–10 ml each of RNAlaterTM and sterile
water. Total RNA from these nematodes was isolated
using three different RNA isolation kits, viz. RNAqueousTM,
RNeasy Plant mini kit and RNAwizTM. RT–PCR
was performed using virus-specific primers for SLRSV
and ArMV. Out of the three RNA isolation kits used,
only RNAaqueousTM gave reproducible results. Nematodes
isolated and stored in RNAlaterTM gave amplification
of expected size (over a period of one month
storage at –20°C) for both the viruses, while nematodes
stored in water did not give any amplification
(even after one week of storage) with RT–PCR. Cucumis
sativus was used as bait plant for confirming the
nematode transmission of these viruses. In the present
study RNAlaterTM was found to be the best nematode
storage solution and RNAqueousTM the best RNA isolation
kit for virus detection in viruliferous nematodes
by RT–PCR.
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| Publisher |
Current Science Association, C V Raman Avenue, PO Box 8005, Bangalore 560 080, India .
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| Date |
2005
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/167/1/AMV.pdf
Kulshrestha, S and Hallan, Vipin and Raikhy, G and Adekunle, O K and Verma, N and Haq, Q M R and Zaidi, A A (2005) Reverse transcription polymerase chain reaction-based detection of Arabis mosaic virus and Strawberry latent ringspot virus in vector nematodes. Current Science, 89 (10). pp. 1759-1762. ISSN 0011-3891 |
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| Relation |
http://ihbt.csircentral.net/167/
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