Complete nucleotide sequence of an Indian isolate of Carnation etched ring virus and its homology with other caulimoviruses.
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Complete nucleotide sequence of an Indian
isolate of Carnation etched ring virus and its
homology with other caulimoviruses.
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| Creator |
Raikhy, G
Hallan, Vipin Kulshrestha, S Raja, Ram Zaidi, A A |
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| Subject |
Plant sciences
Plant viruses |
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| Description |
Carnation etched ring virus (CERV) is a member of
Caulimovirus with double stranded DNA genome of
approx. 8 kb. CERV has been previously characterized
from Holland and the present article is an effort to
completely characterize the Asian isolate of CERV.
Primers were designed for amplification of all genes
encoded by the CERV, viz. movement protein, aphid
transmission factor, DNA binding protein, coat protein,
poly protein and inclusion body matrix genes, including
the complete genome. The CERV genome was sequenced
by amplifying the complete genome of CERV
using primers from 5¢- and 3¢-end and with internal
primers that amplify individual genes and parts of the
genes. The genome was found to comprise of 7924 bp,
with GC content of 37%. The different genes encoded
by CERV were compared with other known CERV
isolates and caulimoviruses. They were found most
conserved with respect to poly protein region (37–65%
amino acid similarity), while least with respect to inclusion
body matrix protein (5–37% amino acid similarity).
CERV isolates from India and Holland when compared
for different genes, showed 96–99% homology
between different genes at the amino acid level. Multiplex
PCR protocols were standardized to amplify
different genes of the CERV genome in one reaction.
Phylogenetic analysis based on different genes indicated
that CERV has independently evolved among caulimoviruses,
but is closely related to Cauliflower mosaic virus.
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| Publisher |
Current Science Association, C V Raman Avenue, PO Box 8005, Bangalore 560 080, India .
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| Date |
2006
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/179/1/Carnation.pdf
Raikhy, G and Hallan, Vipin and Kulshrestha, S and Raja, Ram and Zaidi, A A (2006) Complete nucleotide sequence of an Indian isolate of Carnation etched ring virus and its homology with other caulimoviruses. Current Science, 90 (2). pp. 176-187. ISSN 0011-3891 |
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| Relation |
http://ihbt.csircentral.net/179/
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