High-performance thin layer chromatography method for quantitative determination of four major anthraquinone derivatives in Rheum emodi
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
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| Title |
High-performance thin layer chromatography method for quantitative determination of four major anthraquinone derivatives in Rheum emodi
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| Creator |
Singh, N P
Gupta , A P Sinha, Arun K Ahuja, Paramvir Singh |
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| Subject |
Chemical Engineering
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| Description |
A high-performance thin layer chromatographic (HPTLC) method for the rapid and simple quantification of the four major anthraquinone derivatives i.e. physcion, chrysophanol, emodin and chrysophanol glycoside in Rheum emodi is described. HPTLC of anthraquinone derivatives was performed on pre-coated RP- 18 F-254S HPTLC plates. For achieving good separation, the mobile phase of methanol-water-formic acid (80:19: 1, v/v/v) was used. The densitometric determination of anthraquinone derivatives was carried out at 445 nm in reflection/absorption mode. The calibration curves were linear in the range of 20-100 ng for physcion, 80-400 ng for chrysophanol and emodin, and 200-1000 ng for chrysophanol glycoside. The method was found to be reproducible and convenient for quantitative analysis of anthraquinone derivatives in the methanolic extract of rhizomes of R. emodi collected from three different locations of Western Himalaya, India.
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| Publisher |
Elsevier Science Bv, Po Box 211, 1000 AE Amsterdam, Netherlands
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| Date |
2005
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/253/1/sdarticle7.pdf
Singh, N P and Gupta , A P and Sinha, Arun K and Ahuja, Paramvir Singh (2005) High-performance thin layer chromatography method for quantitative determination of four major anthraquinone derivatives in Rheum emodi. Journal of Chromatography, 1077 (2). pp. 202-206. ISSN 0021-9673 |
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| Relation |
http://ihbt.csircentral.net/253/
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