In vitro micrografting for production of Indian citrus ringspot virus (ICRSV)-free plants of kinnow mandarin (Citrus nobilis Lour X C. deliciosa Tenora)
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
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| Title |
In vitro micrografting for production of Indian citrus ringspot virus (ICRSV)-free plants of kinnow mandarin (Citrus nobilis Lour X C. deliciosa Tenora)
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| Creator |
Singh , Bikram
Sharma, S Rani , G Hallan, Vipin Zaidi, A A Virk, G S Nagpal, A |
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| Subject |
Plant sciences
Plant Biotechnology |
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| Description |
Production of Indian citrus ringspot virus (ICRSV)-free plants from an infected plant of kinnow mandarin (Citrus nobilis Lour 9 C. deliciosa Tenora) is reported. The shoot apices of different sizes (0.2-1.0 mm) excised from the ICRSV-infected plant were micrografted onto decapitated rootstock seedlings of rough lemon (C. jambhiri). Micrograft survival depended on the size of shoot apex and the sucrose concentration of the culture medium. Increase in scion size from 0.2 to 0.7 mm resulted in an increase in micrografting success rate from 30.55 to 51.88%. Further, micrograft survival obtained with 0.2 mm was improved from 30.55 to 38.88% by increasing sucrose concentration in the culture media from 5 to 7.5%. The micrografted plants were tested for ICRSV using ELISA and RT-PCR. All plants raised from 0.2-mm scion were found negative with both ELISA and RT-PCR whereas only 20% of the ELISA negative plants raised from 0.3-mm scion were found negative for ICRSV with RT-PCR. The outcome of this research is the successful establishment, acclimatization and virus testing of micrografted plants.
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| Publisher |
Springer, 233 Spring ST, New York, NY 10013 USA
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| Date |
2008
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
application/pdf |
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| Identifier |
http://ihbt.csircentral.net/313/1/327_2008_In_vitro.pdf
http://ihbt.csircentral.net/313/2/327_2008_In_vitro.pdf Singh , Bikram and Sharma, S and Rani , G and Hallan, Vipin and Zaidi, A A and Virk, G S and Nagpal, A (2008) In vitro micrografting for production of Indian citrus ringspot virus (ICRSV)-free plants of kinnow mandarin (Citrus nobilis Lour X C. deliciosa Tenora). Plant Biotechnology Reports, 2 (2). pp. 137-143. ISSN 1863-5466 |
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| Relation |
http://ihbt.csircentral.net/313/
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