Simple and efficient enzymatic transglycosylation of stevioside by beta-cyclodextrin glucanotransferase from Bacillus firmus.
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Simple and efficient enzymatic transglycosylation of stevioside by beta-cyclodextrin glucanotransferase from Bacillus firmus.
|
|
| Creator |
Jaitak, V
Kaul , V K Kumar, Neeraj Singh , Bikram ---, Bandna Savergave, L S Jogdand, V V Nene, S |
|
| Subject |
Plant Biotechnology
Natural Product Chemistry |
|
| Description |
Stevioside was subjected to 1,4-intermolecular transglycosylation using beta-cyclodextrin glucanotransferase (beta-CGtase) produced from an alkalophilic strain of Bacillus firmus. The reaction was carried out by traditional, ultrasound-assisted and microwave-assisted techniques. Reaction under microwave conditions was faster and was completed in 1 min yielding two 1,4 transglycosylated products, 4'-O-alpha-d-glycosyl stevioside (I) and 4''-O-alpha-d-maltosyl stevioside (II) in 66% and 24%, respectively. The optimum transglycosylation occurred by using stevioside (1.24 mmol), beta-CD (1.76 mmol) and beta-CGtase (2 U/g) under microwave assisted reaction (MAR) in 5 ml sodium phosphate buffer (pH 7) at 50A degrees C and 80 W power. MAR is therefore potentially a useful and economical method for faster transglycosylation of stevioside.
|
|
| Publisher |
Springer, Van Godewijckstraat 30, 3311 GZ Dordrecht, Netherlands
|
|
| Date |
2009
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://ihbt.csircentral.net/334/1/436_2009_Simple_and_Efficent.pdf
Jaitak, V and Kaul , V K and Kumar, Neeraj and Singh , Bikram and ---, Bandna and Savergave, L S and Jogdand, V V and Nene, S (2009) Simple and efficient enzymatic transglycosylation of stevioside by beta-cyclodextrin glucanotransferase from Bacillus firmus. Biotechnology Letters, 31 (9). pp. 1415-20. ISSN 0141-5492 |
|
| Relation |
http://ihbt.csircentral.net/334/
|
|