Immobilization of Lipase by Entrapment in Ca-alginate Beads
IR@IIIM: CSIR-Indian Institute of Integrative Medicine, Jammu
View Archive Info| Field | Value | |
| Title |
Immobilization of Lipase by Entrapment in Ca-alginate Beads
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| Creator |
Bhushan, I.
Parshad, R. Qazi, G.N. Gupta, V.K. |
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| Subject |
Biological Sciences
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| Description |
The lipase-producing strain, Arthrobacter sp. (ABL), isolated was immobilized in Ca-alginate beads by entrapment. The alginate beads were prepared as an aqueous mixture of sodium alginate, the cells and CaCl2 to
increase its reusability, and overall enzyme stability. Various parameters like alginate and CaCl2 concentration, lipase units loading and bead size were
evaluated for optimum immobilization yield. It was observed that with the increase in alginate concentration, the yield of immobilized enzyme also increased up to a limit. A similar pattern was observed with CaCl2 addition;
the optimum concentrations of alginate and CaCl2 observed were 1.5% (w/v) and 2%, respectively. The concentration of enzyme entrapped in the beads with
an activity of 5 units per gram of wet beads was obtained by the addition of 100 units in 10mL of slurry; beyond this amount a very little increase in activity was observed. The maximum immobilization yield was observed with a 1.2mm
bead size; increased bead sizes decreased the yield of immobilization. After optimization of all the parameters, a 40% yield of lipase (ABL) activity was observed in the Ca-alginate beads. These lipase beads were used for 10 cycles for the hydrolysis of triglycerides without any loss in activity. The entrapped lipase was more stable over a wide range of temperatures, pH, and storage time as
compared to free enzyme.
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| Date |
2008
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://iiim.csircentral.net/261/1/10.1177%2D0883911508097866.pdf
Bhushan, I. and Parshad, R. and Qazi, G.N. and Gupta, V.K. (2008) Immobilization of Lipase by Entrapment in Ca-alginate Beads. Journal of Bioactive and Compatible Polymers, 23 (6). pp. 552-562. ISSN 0883-9115 |
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| Relation |
http://dx.doi.org/10.1177/0883911508097866
http://iiim.csircentral.net/261/ |
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