Induction of Mitochondrial-Dependent Apoptosis by an Essential Oil from Tanacetum gracile
IR@IIIM: CSIR-Indian Institute of Integrative Medicine, Jammu
View Archive Info| Field | Value | |
| Title |
Induction of Mitochondrial-Dependent Apoptosis by an Essential Oil from Tanacetum gracile
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| Creator |
Verma, Monika
Singh, Shashank Bhushan, Shashi Pal, Harish Kitchlu, Surinder Koul, Maharaj Thappa, Rajender Saxena, Ajit |
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| Subject |
Biological Sciences
Pharmacological Sciences |
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| Description |
The essential oil of Tanacetum gracile (Accession
no. AT-01 termed AT-01 in the manuscript), a cold desert alpine highly aromatic herb, has 40 constituents including lavendulol (21.5%), lavendulol acetate (1.7 %), α-pinene (11.2%),1,8-cineole (15.2 %), cis-β-ocimene (6.9 %), borneol (6.1%), limonene (5.1%) and chamazulene (3.7 %). AT-01 was evaluated for its anticancer activity. It inhibited HL-60 cell proliferation with an IC50 of 27 μg/
mL. Furthermore, AT-01 induced apoptosis in human
leukemia HL-60 cells as measured by several biological end points. AT-01 induced apoptotic body formation, enhanced annexinV-FITC binding of the cells, increased sub-G0 DNA fraction, loss of mitochondrial membrane potential (Δψmt) and release of cytochrome c from mitochondria, activated
caspase-9 as well as caspase-3, and increased cleavage of PARP in HL-60 cells. Thus, AT-01 induced apoptosis through the mitochondrial dependant pathway in HL-60 cells.
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| Date |
2008
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://iiim.csircentral.net/278/1/10.1055s%2D2008%2D1074503.pdf
Verma, Monika and Singh, Shashank and Bhushan, Shashi and Pal, Harish and Kitchlu, Surinder and Koul, Maharaj and Thappa, Rajender and Saxena, Ajit (2008) Induction of Mitochondrial-Dependent Apoptosis by an Essential Oil from Tanacetum gracile. Planta Medica, 74 (5). pp. 515-520. ISSN 0032-0943 |
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| Relation |
http://dx.doi.org/10.1055/s-2008-1074503
http://iiim.csircentral.net/278/ |
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