Emodin reverses CCl4induced hepatic cytochrome P450 (CYP) enzymatic and ultrastructural changes: Thein vivoevidence
IR@IIIM: CSIR-Indian Institute of Integrative Medicine, Jammu
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| Title |
Emodin reverses CCl4induced hepatic cytochrome P450 (CYP) enzymatic and ultrastructural changes: Thein vivoevidence
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| Creator |
Bhadauria, Monika
Nirala, Satendra Kumar Shrivastava, Sadhana Sharma, Abhilasha Johri, Sonia Chandan, Bal Krishan Singh, Bhupendar Saxena, Ajit Kumar Shukla, Sangeeta |
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| Subject |
Pharmacological Sciences
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| Description |
The curative effect of emodin (1,3,8-trihydroxy-6-
methyl anthraquinone), an active compound of the plant
species Ventilago maderaspatana Gaertn, was evaluated
against carbon tetrachloride (CCl4) induced hepatic cytochrome P450 (CYP) enzymatic and ultrastructural alterations in rats.
Methods: Female rats were administered CCl4 (1.5 mL/kg, ip)
followed by varying doses of emodin (20, 30 and 40 mg/kg,
oral po) after 24 h of CCl4 administration. Animals were euthanized after 24 h of last administration to determine liver function tests in serum, hepatic light microscopic and ultrastructural changes, activity of CYP enzymes, microsomal lipid peroxidation and protein contents, hexobarbitone induced sleep time and bromosulphalein retention.
Results: The CCl4 induced-toxic effects were observed with
sharp elevation in the release of serum transaminases,
alkaline phosphatase, lactate dehydrogenase and g-glutamyl
transpeptidase. An initial study for an optimum dose of
emodin among different dose levels revealed that a 30 mg/kg
dose was effective in restoring all the enzymatic variables and liver histoarchitecture in a dose dependent manner. Exposure to CCl4 diminished the activities of CYP enzymes (i.e. aniline hydroxylase and amidopyrine-N-demethylase and microsomal protein contents with concomitant increase in microsomal lipid peroxidation). Emodin at 30 mg/kg effectively reversed the CCl4 induced hepatotoxic events, which was consistent with ultrastructural observations. Hexobarbitoneinduced sleep time and plasma bromosulphalein retention also improved liver functions after emodin therapy.
Conclusion: By reversal CYP activity and ultrastructural
changes, emodin shows a strong hepatoprotective abilities.
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| Date |
2009
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://iiim.csircentral.net/247/1/10.1111j.1872%2D034X.2008.00380.x.pdf
Bhadauria, Monika and Nirala, Satendra Kumar and Shrivastava, Sadhana and Sharma, Abhilasha and Johri, Sonia and Chandan, Bal Krishan and Singh, Bhupendar and Saxena, Ajit Kumar and Shukla, Sangeeta (2009) Emodin reverses CCl4induced hepatic cytochrome P450 (CYP) enzymatic and ultrastructural changes: Thein vivoevidence. Hepatology Research, 39 (3). pp. 290-300. ISSN 13866346 |
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| Relation |
http://dx.doi.org/10.1111/j.1872-034X.2008.00380.x
http://iiim.csircentral.net/247/ |
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