Immunomodulatory activity of Asparagus racemosus on systemic Th1/Th2 immunity: Implications for immunoadjuvant potential
IR@IIIM: CSIR-Indian Institute of Integrative Medicine, Jammu
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| Title |
Immunomodulatory activity of Asparagus racemosus on systemic Th1/Th2 immunity: Implications for immunoadjuvant potential
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| Creator |
Gautam, Manish
Saha, Santanu Bani, Sarang Kaul, A. Mishra, Sanjay Patil, Dada Satti, N.K. Suri, K.A. Gairola, Sunil Suresh, K. Jadhav, Suresh Qazi, G.N. Patwardhan, Bhushan |
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| Subject |
Pharmacological Sciences
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| Description |
Ethnopharmacological relevance: Roots of Asparagus racemosus Willd (Shatavari in vernacular) are widely
used in Ayurveda as Rasayana for immunostimulation, galactogogue as also in treatment of conditions like ulcers and cancer. Various studies have indicated immunomodulatory properties of Shatavari root extracts and formulations.
Aim of the study: To study the effect of standardized Asparagus racemosus root aqueous extract (ARE) on systemic Th1/Th2 immunity of SRBC sensitized animals.
Materials and methods:We used HPTLC to quantify steroidal saponins (Shatavarin IV, Immunoside®) and flow cytometry to study effects of ARE on Th1/Th2 immunity. SRBC specific antibody titres and DTH responses were also monitored as markers of Th2 and Th1 responses, respectively. We also studied lymphocyte proliferation. Cyclosporin, cyclophosphamide and levamisole were used as controls.
Results: Treatment with ARE (100 mg/(kg b.w. p.o.)) resulted in significant increase of CD3+ and CD4/CD8+
percentages suggesting its effect on T cell activation. ARE treated animals showed significant up-regulation of Th1 (IL-2, IFN-g) and Th2 (IL-4) cytokines suggesting its mixed Th1/Th2 adjuvant activity. Consistent to this, ARE also showed higher antibody titres and DTH responses. ARE, in combination with LPS, Con A or SRBC, produced a significant proliferation suggesting effect on activated lymphocytes.
Conclusion: The study suggests mixed Th1/Th2 activity of ARE supports its immunoadjuvant potential.
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| Date |
2009
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://iiim.csircentral.net/265/1/10.1016j.jep.2008.10.028.pdf
Gautam, Manish and Saha, Santanu and Bani, Sarang and Kaul, A. and Mishra, Sanjay and Patil, Dada and Satti, N.K. and Suri, K.A. and Gairola, Sunil and Suresh, K. and Jadhav, Suresh and Qazi, G.N. and Patwardhan, Bhushan (2009) Immunomodulatory activity of Asparagus racemosus on systemic Th1/Th2 immunity: Implications for immunoadjuvant potential. Journal of Ethnopharmacology, 121 (2). pp. 241-247. ISSN 03788741 |
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| Relation |
http://dx.doi.org/10.1016/j.jep.2008.10.028
http://iiim.csircentral.net/265/ |
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