Isolation of an N-acetyl-d-glucosamine specific lectin from the rhizomes of Arundo donax with antiproliferative activity
IR@IIIM: CSIR-Indian Institute of Integrative Medicine, Jammu
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| Title |
Isolation of an N-acetyl-d-glucosamine specific lectin from the rhizomes of Arundo donax with antiproliferative activity
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| Creator |
Kaur, Amandeep
Singh, Jatinder Kamboj, Sukhdev Singh Sexana, A.K. Pandita, Renu Moti Shamnugavel, M. |
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| Subject |
Chemical Sciences
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| Description |
A lectin with antiproliferative activity towards human cancer cell lines and mitogenic towards human peripheral blood mononuclear cells was purified from the rhizomes of Arundo donax (Linn.) by affinity chromatography on N-acetyl-D-glucosamine linked to epoxy-activated sepharose-6B. The pure preparation apparently yielded a single band of approximately 15 kDa on SDS–PAGE,pH 8.3, under both reducing and non-reducing conditions. The molecular mass of native lectin was 32 kDa as determined by gel filtration chromatography. This showed the lectin to be a dimer, with subunits not held together by disulphide linkages. The A.
donax lectin (ADL) agglutinated rabbit erythrocytes and the agglutination was inhibited by N-acetyl-D-glucosamine and its diand trimer. The lectin was thermostable upto 55 �C and showed optimum activity in the range of pH 7.0–9.0 and comprised of 2.1% carbohydrate content.
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| Date |
2005
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://iiim.csircentral.net/282/1/10.1016j.phytochem.2005.06.026.pdf
Kaur, Amandeep and Singh, Jatinder and Kamboj, Sukhdev Singh and Sexana, A.K. and Pandita, Renu Moti and Shamnugavel, M. (2005) Isolation of an N-acetyl-d-glucosamine specific lectin from the rhizomes of Arundo donax with antiproliferative activity. Phytochemistry, 66 (16). pp. 1933-1940. ISSN 00319422 |
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| Relation |
http://dx.doi.org/10.1016/j.phytochem.2005.06.026
http://iiim.csircentral.net/282/ |
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