Effects of Extraction Methods on Phenolic Contents and Antioxidant Activity in Aerial Parts of Potentilla atrosanguinea Lodd. and Quantification of Its Phenolic Constituents by RP-HPLC†
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Effects of Extraction Methods on Phenolic Contents
and Antioxidant Activity in Aerial Parts of Potentilla
atrosanguinea Lodd. and Quantification of Its
Phenolic Constituents by RP-HPLC†
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| Creator |
Kalia, K
Sharma, K Singh, H P Singh , Bikram |
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| Subject |
Nutraceuticals
Natural Product Chemistry |
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| Description |
The effects of different solvent systems (methanol, ethanol, acetone, and their 50% aqueous
concentrations) and extraction procedures (microwave, ultrasound, Soxhlet and maceration) on the
antioxidant activity of aerial parts of Potentilla atrosanguinea were investigated by three different
bioassays: 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2′-
diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays and ferric reducing antioxidant potential
(FRAP). The 50% aqueous ethanol extracts exhibited strong antioxidant activity measured in terms
of Trolox equivalent antioxidant capacity (TEAC) [(54.34 to 122.96, 29.82 to 101.22 and 13.64 to
41.43) mg of Trolox/g] with ABTS•+, DPPH• and FRAP assays, respectively. In general, TEAC of
Soxhlet extracts was found to be 1.8 and 3 times higher than ultrasound and maceration but slightly
(1.2 times) higher than microwave. A positive correlation (r2 ) 0.931 to 0.982) was observed between
total polyphenol (TPC) and total flavonoid (TFC) contents which ranged between 26.7 to 30.7 mg/g
gallic acid equivalent and 16.8 to 20.8 mg/g quercetin equivalent respectively, with antioxidant activity.
In addition, some of its bioactive phenolic constituents which contribute largely toward antioxidant
potential such as chlorogenic acid, catechin, caffeic acid, p-coumaric acid and quercetin were also
quantified in different extracts by RP-HPLC.
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| Date |
2008
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/125/1/2008_Effects.pdf_10129%2D10134.pdf
Kalia, K and Sharma, K and Singh, H P and Singh , Bikram (2008) Effects of Extraction Methods on Phenolic Contents and Antioxidant Activity in Aerial Parts of Potentilla atrosanguinea Lodd. and Quantification of Its Phenolic Constituents by RP-HPLC†. Journal of Agricultural and Food Chemistry, 56 (21). pp. 10129-10134. |
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| Relation |
http://ihbt.csircentral.net/125/
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