An efficient method for explant sterilization for reduced contamination
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
An efficient method for explant sterilization for reduced contamination
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| Creator |
Thakur, Rajesh
Sood, A |
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| Subject |
Plant sciences
Plant Biotechnology |
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| Description |
An improved method for sterilization of explants was tested and found to be suitable for plants with
elongated internodes, such as bamboos. Final cutting of the explants into single nodal segments for
inoculation was done only after surface sterilization of multi-nodal explants in a stoppered glass measuring
cylinder. This minimized penetration of the contaminants and the sterilizing agents into the exposed
intercellular spaces and vascular cavities at the cut ends, thereby minimizing their harmful effects. The
method was experimented upon three different plants, viz., bamboo, tea and rose. Through this method the
number of cultures getting contaminated was substantially reduced as compared to the conventional means
where single nodal explants were used, employing identical treatments. Moreover, in this method, the
number of cultures showing bud-break also showed a marked increase thereby resulting in a tremendous
increase in the percentage of successfully established proliferating cultures
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| Date |
2006
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/200/1/effect.pdf
Thakur, Rajesh and Sood, A (2006) An efficient method for explant sterilization for reduced contamination. Plant Cell, Tissue and Organ Culture, 84. pp. 369-371. |
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| Relation |
http://ihbt.csircentral.net/200/
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