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Differential expression of Histone H3 gene in tea (Camellia sinensis (L.) O. Kuntze) suggests its role in growing tissue

IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur


Field	Value

Title	Differential expression of Histone H3 gene in tea (Camellia sinensis (L.) O. Kuntze) suggests its role in growing tissue

Creator	Singh, K Kumar, Sanjay Ahuja, Paramvir Singh

Subject	Plant sciences Nutraceuticals

Description	Histone proteins are integral part of chromatin and their expression is typically linked toDNAreplication in the S phase of cell cycle. Histone H3 is one of the four histones, along with H2A, H2B and H4, which forms the eukaryotic nucleosome octomer core. Using differential display of mRNA and rapid amplification of cDNA ends (RACE), a full-length Histone H3.1 cDNA (CsH3) was isolated from tea leaves. The open reading frame consisted of 411 nucleotides and deduced amino acid sequence comprised of 136 amino acid residues. CsH3 shared 79–82% and 98% identity at nucleotide and amino acid sequences, respectively with Histone H3 isolated from other plant species. During active-growth period of tea, higher expression was observed in apical buds that decreased gradually with increasing age of the leaf. During dormancy season, the expression of CsH3 was severely down-regulated in all the leaves studied. CsH3 was found to be down regulated in response to drought stress and ABA treatment and upregulated by GA3 treatment. A positive association of CsH3 abundance with active cellular growth suggested its role in plant growth and development.

Date	2009

Type	Article PeerReviewed

Format	application/pdf

Identifier	http://ihbt.csircentral.net/222/1/mol.pdf Singh, K and Kumar, Sanjay and Ahuja, Paramvir Singh (2009) Differential expression of Histone H3 gene in tea (Camellia sinensis (L.) O. Kuntze) suggests its role in growing tissue. Molecular Biology Reports, 36 (3). pp. 537-542.

Relation	http://ihbt.csircentral.net/222/