Simultaneous densitometric determination of artemisinin, artemisinic acid and arteannuin-B in Artemisia annua using reversed-phase thin layer chromatography
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Simultaneous densitometric determination of
artemisinin, artemisinic acid and arteannuin-B in
Artemisia annua using reversed-phase thin layer
chromatography
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| Creator |
Bhandari, P
Singh , Bikram Gupta , A P Kaul , V K |
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| Subject |
Natural Product Chemistry
Information and Science Management |
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| Description |
A rapid and simple RP-TLC method for simultaneous quantification of pharmacologically
important sesquiterpene artemisinin (AM) together with its precursors arteannuin-
B (AB) and artemisinic acid (AA) in the inflorescence part of Artemisia annua
plant has been developed. The RP-TLC of sesquiterpenes was performed on RP-18
F254 S thin-layer chromatographic plates by developing in mobile phase, containing
0.2% TFA in water/ACN (35 : 65, v/v). The densitometric determination of AM, AB and
AA was carried out after derivatization with anisaldehyde reagent at 426 nm in
absorption-reflectance mode.
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| Date |
2005
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/256/1/sep01.pdf
Bhandari, P and Singh , Bikram and Gupta , A P and Kaul , V K (2005) Simultaneous densitometric determination of artemisinin, artemisinic acid and arteannuin-B in Artemisia annua using reversed-phase thin layer chromatography. Journal of Separation Science, 28. pp. 2288-2292. |
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| Relation |
http://ihbt.csircentral.net/256/
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