Expression analysis of a stress-modulated transcript in drought tolerant and susceptible cultivars of sorghum (Sorghum bicolor)
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Expression analysis of a stress-modulated
transcript in drought tolerant and susceptible
cultivars of sorghum (Sorghum bicolor)
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| Creator |
Sharma, A D
Kumar, Sanjay Singh, P |
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| Subject |
Plant Biotechnology
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| Description |
The present study reports the cloning of a 581 bp sequence, designated as SbEST8,
from the osmotically stressed germinated seeds of a drought tolerant cultivar of
sorghum (Sorghum bicolor). The SbEST8, which shows no homology with the reported
gene sequences, is present in multiple copies and lacks restriction fragment length
polymorphism among different sorghum cultivars. The expression of SbEST8 in the
germinating seeds of sorghum was modulated by different abiotic stresses. Kinetic
studies revealed that imposition of osmotic stress after 8 h resulted in maximum levels
of SbEST8 mRNA in the germinating seeds of cv. ICSV-272, with further stress causing a
decline to undetectable levels by 16 h. However, relieving the stress after 12 h
resulted in an enhancement of SbEST8 mRNA levels for at least another 4 h following
which it declined. The decrease in SbEST8 mRNA levels in the leaves at 30 DAS in
response to drought stress was observed only in the drought susceptible cultivar (CSV-
216), whereas its expression was either increased substantially or remained
unaffected in the tolerant cultivars, thus suggesting its role in water stress tolerance.
& 2005 Elsevier GmbH. All rights reserved.
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| Date |
2006
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/260/1/sorghum.pdf
Sharma, A D and Kumar, Sanjay and Singh, P (2006) Expression analysis of a stress-modulated transcript in drought tolerant and susceptible cultivars of sorghum (Sorghum bicolor). Journal of Plant Physiology, 163 (5). pp. 570-576. |
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| Relation |
http://ihbt.csircentral.net/260/
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