Production of Cucumber mosaic virus-free chrysanthemums by meristem tip culture.
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Production of Cucumber mosaic virus-free chrysanthemums by
meristem tip culture.
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| Creator |
Verma, N
Raja, Ram Hallan, Vipin Kumar, K Zaidi, A A |
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| Subject |
Plant sciences
Plant viruses |
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| Description |
Chrysanthemum cv. Regol Time was found infected with a viral disease showing characteristic chlorotic spots on leaves and
stunting of plants. The virus was identified as Cucumber mosaic cucumovirus (CMV) on the basis of mechanicalinocul ation on
Chenopodium amaranticolor and Cucumis sativus, DAS-ELISA and RT-PCR. CMV was eliminated from chrysanthemum plants
using meristem tip culture. MS medium amended with BAP (2 mg/l) and IBA (0.05 mg/l) was used for shoot proliferation and IBA
(0.05 mg/l) for rooting of the plants. Virus-free plants (84%) were obtained from the optimum size (0.3 mm) of meristem tips (as
indexed by DAS-ELISA). Of these, 72% plants were found negative for CMV as indexed by RT-PCR. Virus indexing by RT-PCR
was found to be a reliable method.
r 2003 Elsevier Ltd. All rights reserved.
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| Publisher |
Elsevier Science Ltd., The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England .
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| Date |
2004
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/379/1/111_A_2004_Production.pdf
Verma, N and Raja, Ram and Hallan, Vipin and Kumar, K and Zaidi, A A (2004) Production of Cucumber mosaic virus-free chrysanthemums by meristem tip culture. Crop Protection, 23 (5). pp. 469-473. ISSN 0261-2194 |
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| Relation |
http://ihbt.csircentral.net/379/
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