A rapid determination of podophyllotoxin in podophyllum hexandrum by reverse phase high performance thin layer chromatography
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
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| Title |
A rapid determination of podophyllotoxin in podophyllum hexandrum by reverse phase high performance thin layer chromatography
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| Creator |
Mishra, Nidhi
Gupta , A P Singh , Bikram Kaul , V K Ahuja, Paramvir Singh |
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| Subject |
Natural Product Chemistry
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| Description |
A sensitive, selective, and precise RP-HPTLC method has been developed for the quantification of podophyllotoxin in Podophyllum hexandrum, collected from different locations of the western Himalaya of India, ranging from 3250 to 4100 meters above the mean sea level. The assay employed RP-18 F-254 TLC plates (chromatoplates) as the stationary phase. The solvent system consisted of acetonitrile-water (4: 6, v/v) and gave better resolution, with well separated, compact spots for podophyllotoxin (Rf value 0.41 +/- 0.02). Densitometric analysis was carried out in the absorption remission mode at 217 nm.
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| Publisher |
Taylor & Francis, Inc., 325 Chestnut Street, Suite 800, Philadelphia, PA 19106
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| Date |
2005
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/400/1/145_2005_A_Rapid.pdf
Mishra, Nidhi and Gupta , A P and Singh , Bikram and Kaul , V K and Ahuja, Paramvir Singh (2005) A rapid determination of podophyllotoxin in podophyllum hexandrum by reverse phase high performance thin layer chromatography. Journal of Liquid Chromatography & Related Technologies, 28 (5). pp. 677-691. ISSN 1082-6076 |
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| Relation |
http://ihbt.csircentral.net/400/
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