Leishmania Donovani: Dyskinetoplastid Cells Survive and Proliferate in the Presence of Pyruvate and Uridine but do not Undergo Apoptosis after Treatment with Camptothecin.
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Leishmania Donovani: Dyskinetoplastid Cells Survive and
Proliferate in the Presence of Pyruvate and Uridine but do not Undergo Apoptosis after Treatment with Camptothecin.
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| Creator |
Sen, Nilkantha
Banerjee, Bijoylaxmi Gupta, Souvik Sen Das, Benu Brata Ganguly, Agneyo Majumder, Hemanta K |
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| Subject |
Infectious Diseases and Immunology
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| Description |
We have shown that treatment with luteolin in leishmanial cells causes loss of mt-DNA and induces apoptosis through mitochondria dependent pathway [Sen, N., Das, B.B., Ganguly, A., Banerjee, B., Sen, T., Majumder, H.K., 2006. Leishmania donovani: intracellular ATP level regulates apoptosis-like death in luteolin induced dyskinetoplastid cells. Experimental Parasitology, in press]. Here, we report that mitochondrial DNA depleted leishmanial cells require exogenous sources of pyruvate and uridine to survive and proliferate. The presence of pyruvate and uridine in a growing media help them to produce suYcient amount of glycolytic ATP to maintain the mitochondrial
membrane potential in the absence of their functional ETC. Treatment of wild type cells with CPT causes generation of ROS that leads to apoptosis. But unlike the normal cells ROS was not generated in these mt-DNA depleted cells after treatment with CPT. Taken together we have shown for the Wrst time that dyskinetoplastid cells are auxotrophic for pyruvate and uridine and apoptosis cannot be induced in
these cells in the presence of CPT. Therefore, the presence of mitochondrial DNA is absolutely necessary for the cytotoxicity of CPT in kinetoplastid parasites.
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| Date |
2007
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1126/1/EXPERIMENTAL_PARASITOLOGY___115_(2)_215%2D219;2007[121].pdf
Sen, Nilkantha and Banerjee, Bijoylaxmi and Gupta, Souvik Sen and Das, Benu Brata and Ganguly, Agneyo and Majumder, Hemanta K (2007) Leishmania Donovani: Dyskinetoplastid Cells Survive and Proliferate in the Presence of Pyruvate and Uridine but do not Undergo Apoptosis after Treatment with Camptothecin. Experimental Parasitology, 115 (2). pp. 215-219. |
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| Relation |
http://dx.doi.org/10.1016/j.exppara.2006.08.005
http://www.eprints.iicb.res.in/1126/ |
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