Purification and Characterization of a Low Molecular Weight Multifunctional Cytotoxic Phospholipase A2 from Russell’s Viper Venom.
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Purification and Characterization of a Low Molecular Weight Multifunctional Cytotoxic Phospholipase A2 from Russell’s Viper Venom.
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| Creator |
Maity, Gargi
Mandal, Somnath Chatterjee, Amitabha Bhattacharyya, Debasish |
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| Subject |
Structural Biology & Bioinformatics
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| Description |
A basic toxin from Russell’s viper venom of 7.2 kDa (RVV-7) has been purified to homogeneity after partial unfolding by 4M urea followed by
filtration through Centricon-30 membrane. Its N-terminal sequence showed strong homology with snake venom cytotoxins. Cytotoxic activity of
RVV-7 has been demonstrated with B16F10 melanoma cells. PLA2 activity was observed in cytotoxin (CX3) from Naja kauthia bearing sequence
homology with RVV-7. Phospholipase A2 and trypsin inhibitory activities were also observed with RVV-7. Chemical modification and inhibition
studies suggested independent functional sites for these activities. A qualitative assessment of tumor growth inhibition by RVV-7 has been made.
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| Date |
2007
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1138/1/JOURNAL_OF_CHROMATOGRAPHY_B%2DANALYTICAL_TECHNOLOGIES_IN_THE_BIOMEDICAL_AND_LIFE_SCIENCES__845(2)_Pages_232%2D243;_2007[123].pdf
Maity, Gargi and Mandal, Somnath and Chatterjee, Amitabha and Bhattacharyya, Debasish (2007) Purification and Characterization of a Low Molecular Weight Multifunctional Cytotoxic Phospholipase A2 from Russell’s Viper Venom. Journal of Chromatography B, 845. pp. 232-243. |
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| Relation |
http://dx.doi.org/10.1016/j.jchromb.2006.08.038
http://www.eprints.iicb.res.in/1138/ |
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