Viper and Cobra Venom Neutralization by b-Sitosterol and Stigmasterol Isolated from the Root Extract of Pluchea Indica Less. (Asteraceae)
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Viper and Cobra Venom Neutralization by b-Sitosterol and Stigmasterol Isolated from the Root Extract of Pluchea Indica Less. (Asteraceae)
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| Creator |
Gomes, Aparna
Saha, Archita Chatterjee, Ipshita Chakravarty, A K |
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| Subject |
Chemistry
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| Description |
We reported previously that the methanolic root extract of the Indian medicinal plant Pluchea indica Less.
(Asteraceae) could neutralize viper venom-induced action [Alam, M.I., Auddy, B., Gomes, A., 1996. Viper venom
neutralization by Indian medicinal plant (Hemidesmus indicus and P. indica) root extracts. Phytother. Res. 10, 58–61].
The present study reports the neutralization of viper and cobra venom by b-sitosterol and stigmasterol isolated from
the root extract of P. indica Less. (Asteraceae). The active fraction (containing the major compound b-sitosterol and
the minor compound stigmasterol) was isolated and purified by silica gel column chromatography and the structure
was determined using spectroscopic analysis (EIMS, 1H NMR, 13C NMR). Anti-snake venom activity was studied in
experimental animals. The active fraction was found to significantly neutralize viper venom-induced lethal,
hemorrhagic, defibrinogenation, edema and PLA2 activity. Cobra venom-induced lethality, cardiotoxicity,
neurotoxicity, respiratory changes and PLA2 activity were also antagonized by the active component. It potentiated
commercial snake venom antiserum action against venom-induced lethality in male albino mice. The active fraction
could antagonize venom-induced changes in lipid peroxidation and superoxide dismutase activity. This study suggests
that b-sitosterol and stigmasterol may play an important role, along with antiserum, in neutralizing snake venominduced
actions.
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| Date |
2007
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1150/1/PHYTOMEDICINE__14_(9)_637%2D643_;2007[45].pdf
Gomes, Aparna and Saha, Archita and Chatterjee, Ipshita and Chakravarty, A K (2007) Viper and Cobra Venom Neutralization by b-Sitosterol and Stigmasterol Isolated from the Root Extract of Pluchea Indica Less. (Asteraceae). Phytomedicine, 14 (9). pp. 637-643. |
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| Relation |
http://dx.doi.org/10.1016/j.phymed.2006.12.020
http://www.eprints.iicb.res.in/1150/ |
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