A Crystalline Compound (BM-ANF1) from the Indian Toad (Bufo melanostictus, Schneider) Skin Extract, Induced Antiproliferation and Apoptosis in Leukemic and Hepatoma Cell Line Involving Cell Cycle Proteins.
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
A Crystalline Compound (BM-ANF1) from the Indian Toad (Bufo
melanostictus, Schneider) Skin Extract, Induced Antiproliferation and Apoptosis in Leukemic and Hepatoma Cell Line Involving Cell Cycle Proteins.
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| Creator |
Gomes, Antony
Giri, Biplab Kole, Labanyamoy Saha, Archita Debnath, Anindita Gomes, Aparna |
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| Subject |
Drug Development/Diagnostics & Biotechnology
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| Description |
In our earlier communication, it was reported that Indian toad (Bufo melanostictus) skin extract (TSE) possesses
antiproliferative and apoptogenic activity in U937 and K562 cells [Giri et al., 2006. Antiproliferative, cytotoxic and
apoptogenic activity of Indian toad (Bufo melanostictus, Schneider) skin extract in U937 and K562 cells. Toxicon 48 (4),
388–400]. In the present study, a compound (BM-ANF1) has been isolated from the TSE by alumina gel column
chromatography, crystallized and evaluated for its antiproliferative and apoptogenic activity in U937, K562 and HepG2
cells. BM-ANF1 produced dose-dependent inhibition of U937, K562 and HepG2 cell growth. The antiproliferative activity
was reflected by the MTT assay and demonstrated by the reduced expression of proliferative cell nuclear antigen (PCNA).
Flow-cytometric analysis showed that BM-ANF1 arrested the cell cycle at G1 phase and enhanced annexin-V binding in
U937 and K562 cells. Scanning electron microscopic and fluorescent microscopic analysis of U937 and K562 cells revealed
the apoptogenic nature of the compound. Alkaline comet assay showed that BM-ANF1 produced DNA fragmentation.
The dose-dependent expression of caspase 3 indicated that the apoptogenic properties of BM-ANF1 were mediated
through the activation of downstream effector nucleases in the cancer cells. The increased expression of p53 and moderate
expression of p21Cip1/p27Kip1 due to BM-ANF1 treatment in HepG2 cells supported that the apoptogenic activity of
BM-ANF1 was mediated through p53 tumor-suppressor gene expression followed by the expression of p21Cip1 and p27
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| Date |
2007
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1159/1/TOXICON___50_(6)__835%2D849;2007[20].pdf
Gomes, Antony and Giri, Biplab and Kole, Labanyamoy and Saha, Archita and Debnath, Anindita and Gomes, Aparna (2007) A Crystalline Compound (BM-ANF1) from the Indian Toad (Bufo melanostictus, Schneider) Skin Extract, Induced Antiproliferation and Apoptosis in Leukemic and Hepatoma Cell Line Involving Cell Cycle Proteins. Toxicon, 50 (6). pp. 835-849. |
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| Relation |
http://dx.doi.org/10.1016/j.toxicon.2007.06.018
http://www.eprints.iicb.res.in/1159/ |
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