Cholesterol biosensor based on electrochemically prepared polyaniline conducting polymer film in presence of a nonionic surfactant
IR@NEIST: CSIR-North East Institute of Science and Technology, Jorhat
View Archive Info| Field | Value | |
| Title |
Cholesterol biosensor based on electrochemically
prepared polyaniline conducting polymer film
in presence of a nonionic surfactant
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| Creator |
KHAN , RAJU
Solanki, Pratima R. Kaushik, Ajeet Singh, S P Ahmed, Sharif Malhotra, B D |
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| Subject |
Analytical Chemistry
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| Description |
Cholesterol biosensor has been fabricated by
covalently coupling cholesterol oxidase (ChOx) via glutaraldehyde
onto electrochemically prepared polyaniline film
in presence of TritonX-100 [4-(1,1,3,3-tetramethylbutyl)
phenyl polyethylene glycol], a non-ionic surfactant onto
indium-tin-oxide (ITO) glass substrate. These ChOx/PANITX-
100/ITO bioelectrodes have been characterized using
Fourier transform infrared (FTIR) spectroscopy, cyclic
voltammetry (CV) and scanning electron microscopy
(SEM) techniques. The results of response measurements
carried out on ChOx/PANI-TX-100/ITO bioelectrodes
using amperometric and photometric techniques, reveal
detection limit as 5 mg/dl, linearity from 5 to 400 mg/dl of
cholesterol and sensitivity as 131 μA/(mg/dl cm−2). These
biosensing electrodes are thermally stable up to 65 °C, can
be used about 20 times and have a shelf-life of about 10
weeks when stored at 4 °C. Attempts have also been made
to utilize the ChOx/PANI-TX-100/ITO bioelectrodes for
estimation of free cholesterol concentration in serum
samples.
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| Publisher |
Springer
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| Date |
2009
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://neist.csircentral.net/215/1/2720_JPR2009_16p363%E2%80%93373.pdf
KHAN , RAJU and Solanki, Pratima R. and Kaushik, Ajeet and Singh, S P and Ahmed, Sharif and Malhotra, B D (2009) Cholesterol biosensor based on electrochemically prepared polyaniline conducting polymer film in presence of a nonionic surfactant. J Polymer Research, 16. pp. 363-373. |
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| Relation |
http://neist.csircentral.net/215/
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