PCR based molecular characterization of Nepenthes khasiana Hook. f.—pitcher plant
IR@NEIST: CSIR-North East Institute of Science and Technology, Jorhat
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| Title |
PCR based molecular characterization of Nepenthes
khasiana Hook. f.—pitcher plant
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| Creator |
Bhau, B S
Medhi, K Sarkar, T SAIKIA , S P |
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| Subject |
Plant Science
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| Description |
Nepenthes khasiana Hook. f. belonging to
monotypic family Nepenthaceae is a rare, endangered,
dioecious member of the carnivorous plant
found in North-East India. The plant is endemic to the
Indian state of Meghalaya and is distributed throughout
the state from West Khasi hills to East Khasi hills,
Jaintia hills and East, West to South Garo hills from
1,000 to ca. 1,500 m altitude. Multi-locus analysis
using PCR based Random Amplified Polymorphic
DNA (RAPD) and Inter Simple Sequence Repeats
(ISSR) markers were used for the first time to assess
the genetic diversities of N. khasiana Hook. f.
collected from different parts of Meghalaya. It was
observed that RAPD analysis showed more polymorphism
than ISSR fingerprinting in revealing genetic
polymorphism in N. khasiana Hook. f. The result of
cluster analysis by using UPGMA method showed
that the groups based on pooled RAPD–ISSR genetic
similarity were more similar than the groups based on
RAPD. Furthermore, genetic similarity reveals variability
within the population at Jarain of Jaintia hills,
while between populations the Baghmara region differs from the others with at least 40% dissimilarity.
The results show a broad range of genetic diversity
within the populations of N. khasiana Hook. f.
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| Publisher |
Springer
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| Date |
2009
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://neist.csircentral.net/217/1/2723Genetic_Resources_and_Crop_Evolution_2009.pdf
Bhau, B S and Medhi, K and Sarkar, T and SAIKIA , S P (2009) PCR based molecular characterization of Nepenthes khasiana Hook. f.—pitcher plant. Genetic Resources and Crop Evolution, 56 (8). pp. 1183-1193. |
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| Relation |
http://neist.csircentral.net/217/
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