Genetic Diversity of Plant Growth Promoting Rhizobacteria Isolated from Rhizospheric Soil of Wheat Under Saline Condition
IR@NEIST: CSIR-North East Institute of Science and Technology, Jorhat
View Archive Info| Field | Value | |
| Title |
Genetic Diversity of Plant Growth Promoting Rhizobacteria
Isolated from Rhizospheric Soil of Wheat Under Saline Condition
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| Creator |
Upadhyay, Sudhir K.
Singh, Devendra P. SAIKIA , R |
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| Subject |
Biotechnology
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| Description |
In this study, a total of 130 rhizobacteria was
isolated from a saline infested zone of wheat rhizosphere,
and screened for plant growth promoting (PGP) traits at
higher salt (NaCl) concentrations (2, 4, 6, and 8%). The
results revealed that 24 rhizobacterial isolates were tolerant
at 8% NaCl. Although all the 24 salt tolerable isolates
produced indole-3-acetic acid (IAA), while 10 isolates
solubilized phosphorus, eight produced siderophore, and
six produced gibberellin. However, only three isolates
showed the production of 1-aminocyclopropane-1-carboxylate
(ACC) deaminase. Diversity was analyzed through
16S rDNA-RFLP, and of these isolates with three tetra
cutter restriction enzymes (HaeIII, AluI, and MspI), the
representative cluster groups were identified by 16S rDNA
sequencing. Bacillus and Bacillus-derived genera were
dominant which showed PGP attributes at 8% NaCl concentration.
Out of 24 isolates, nitrogen fixing ability (nif H
gene) was detected in the two isolates, SU18 (Arthrobacter
sp.) and SU48.
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| Publisher |
Springer
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| Date |
2009
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://neist.csircentral.net/232/1/2757_Curr_Microbiol.pdf
Upadhyay, Sudhir K. and Singh, Devendra P. and SAIKIA , R (2009) Genetic Diversity of Plant Growth Promoting Rhizobacteria Isolated from Rhizospheric Soil of Wheat Under Saline Condition. Current Microbiology, 59 (5). pp. 489-496. |
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| Relation |
http://neist.csircentral.net/232/
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