Protection From Radiation-InducedMitochondrial and Genomic DNA Damage by an Extract of Hippophae rhamnoides
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Protection From Radiation-InducedMitochondrial
and Genomic DNA Damage by an Extract of
Hippophae rhamnoides
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| Creator |
Shukla, S K
Chaudhary, P Kumar, I P Samanta, N Afrin, F Gupta, M L Sharma , U K Sinha, Arun K Sharma, Y K Sharma, R K |
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| Subject |
Plant sciences
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| Description |
Hippophae rhamnoides or seabuckthorn is used
extensively in Indian and Tibetan traditional medicine
for the treatment of circulatory disorders, ischemic
heart disease, hepatic injury, and neoplasia. In
the present study, we have evaluated the radioprotective
potential of REC-1001, a fraction isolated
from the berries of H. rhamnoides. Chemical analysis
of the extract indicated that REC-1001 was
*68% by weight polyphenols, and contained
kaempferol, isorhamnetin, and quercetin. The effect
of REC-1001 on modulating radiation-induced DNA
damage was determined in murine thymocytes by
measuring nonspecific nuclear DNA damage at the
whole genome level using the alkaline halo assay
and by measuring sequence/gene-specific DNA
damage both in nuclear DNA (b-globin gene) and in
mitochondrial DNA using a quantitative polymerase
chain reaction. Treatment with 10 Gy resulted in a
significant amount of DNA damage in the halo
assay and reductions in the amplification of both the
b-globin gene and mitochondrial DNA. REC-1001
dose-dependently reduced the amount of damage
detected in each assay, with the maximum protective
effects observed at the highest REC-1001 dose evaluated
(250 lg/ml). Studies measuring the nicking of
naked plasmid DNA further established the radioprotective
effect of REC-1001. To elucidate possible
mechanisms of action, the antioxidant properties
and the free-radical scavenging activities of REC-
1001 were evaluated. REC-1001 dose-dependently
scavenged radiation-induced hydroxyl radicals,
chemically-generated superoxide anions, stabilized
DPPH radicals, and reduced Fe3þ to Fe2þ. The results
of the study indicate that the REC-1001 extract of H.
rhamnoides protects mitochondrial and genomic
DNA from radiation-induced damage. The polyphenols/
flavonoids present in the extract might be responsible
for the free radical scavenging and DNA protection
afforded by REC-1001. Environ. Mol. Mutagen.
47:647–656, 2006.
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| Date |
2006
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/215/1/hippo.pdf
Shukla, S K and Chaudhary, P and Kumar, I P and Samanta, N and Afrin, F and Gupta, M L and Sharma , U K and Sinha, Arun K and Sharma, Y K and Sharma, R K (2006) Protection From Radiation-InducedMitochondrial and Genomic DNA Damage by an Extract of Hippophae rhamnoides. Environmental and Molecular Mutagenesis, 47. pp. 647-656. |
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| Relation |
http://ihbt.csircentral.net/215/
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