Phenylalanine ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H) and catechins (flavan-3-ols) accumulation in tea
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Phenylalanine ammonia-lyase (PAL) and cinnamate
4-hydroxylase (C4H) and catechins (flavan-3-ols)
accumulation in tea
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| Creator |
Singh, K
Kumar, Sanjay Rani, A Gulati, Ashu Ahuja, Paramvir Singh |
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| Subject |
Plant Biotechnology
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| Description |
Phenylalanine ammonia-lyase and cinnamate 4-
hydroxylase are important enzymes in allocating significant
amounts of carbon from phenylalanine into the biosynthesis
of several important secondary metabolites. Tea is an
important crop of commerce known for its beverage and
medicinally important flavonoid compounds, mainly catechins.
As metabolic flux for the operation of the flavonoid
pathway is maintained through the activities of PAL and
C4H, thus, catechins biosynthesis in tea is critically
dependent on the products of these enzymes. We examined
the expression of PAL and C4H. Sequence encoding CsPAL
was isolated from tea by polymerase chain reaction using
sequence information available at the NCBI GenBank.
Sequence encoding C4H was isolated from tea by using
differential display of mRNA and rapid amplification of
cDNA ends technology. CsC4H (AY641731) comprised
of 1,352 bp full-length cDNA with open reading frame of
1,173 bp encoding 390 amino acids. Catechin contents
decreased in response to drought stress (DS), abscisic acid
(ABA), and gibberellic acid (GA3) treatments but increased
in response to wounding. The expression of CsPAL and
CsC4H showed the same behavior under the above treatments
and was also in accordance with the catechin
contents. A positive correlation between catechin contents
and gene expression suggested a critical role of the
enzymes in catechins biosynthesis and a crosstalk between
phenylpropanoid and flavonoid pathways.
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| Date |
2009
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/504/1/297_2009_2%2D12%2D2011_Phenylalanine.pdf
Singh, K and Kumar, Sanjay and Rani, A and Gulati, Ashu and Ahuja, Paramvir Singh (2009) Phenylalanine ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H) and catechins (flavan-3-ols) accumulation in tea. Functional and Integrative Genomics, 9 (1). pp. 125-134. |
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| Relation |
http://ihbt.csircentral.net/504/
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