Cadmium Induced Oxidative Stress Influence on Glutathione Metabolic Genes of Camellia sinensis (L.) O. Kuntze
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Cadmium Induced Oxidative Stress Influence on
Glutathione Metabolic Genes of Camellia
sinensis (L.) O. Kuntze
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| Creator |
Mohanpuria, P
Rana, N K Yadav , S K |
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| Subject |
Plant sciences
Plant Biotechnology |
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| Description |
Glutathione, a tripeptide with sulfhydryl (-SH) group is a very crucial compound primarily
involved in redox balance maintenance of the cellular environment. In this study, we monitored the influence
of Cd exposure on the transcript levels of glutathione metabolic genes in bud tissues, the youngest
leaf, of Camellia sinensis L. In addition, some physiochemical parameters were also studied. Cd exposure
decreased chlorophyll and protein contents, while increase was observed in lipid peroxidation upon Cd
treatments. These changes were found to be concentration and duration dependent, indicating the occurrence
of oxidative stress upon Cd exposure. The transcript levels of glutathione biosynthetic genes viz. �-
glutamylcysteine synthetase (�-ECS) and glutathione synthetase (GSHS) increased upon Cd exposure.
Furthermore, transcript levels of glutathione reductase (GR), an enzyme involved in reduction of oxidized
glutathione (GSSG) to reduced glutathione (GSH), also showed upregulation on Cd exposure. However,
the transcript levels of glutathione-S-transferase (GST), an enzyme involved in forming metal–GSH complex
and help in sequestration of high levels of metal ions to vacuole, did not show any change on Cd
treatment. This study document that Cd exposure induces oxidative stress in Camellia sinensis and the
upregulation in transcript levels of glutathione metabolic genes except GST have suggested the role of
these enzymes in the protection of plants from high level Cd exposure. # 2007 Wiley Periodicals, Inc. Environ
Toxicol 22: 368–374, 2007.
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| Date |
2007
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/523/1/379_2%2D12%2D2011.pdf
Mohanpuria, P and Rana, N K and Yadav , S K (2007) Cadmium Induced Oxidative Stress Influence on Glutathione Metabolic Genes of Camellia sinensis (L.) O. Kuntze. Environmental Toxicology, 22. pp. 368-374. |
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| Relation |
http://ihbt.csircentral.net/523/
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