Early low-temperature responsive mitogen activated protein kinases RaMPK1 and RaMPK2 from Rheum australe D. Don respond differentially to diverse stresses
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Early low-temperature responsive mitogen activated protein
kinases RaMPK1 and RaMPK2 from Rheum australe D. Don
respond differentially to diverse stresses
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| Creator |
Ghawana, S
Kumar, Sanjay Ahuja, Paramvir Singh |
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| Subject |
Plant sciences
Plant Biotechnology |
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| Description |
Rheum grows luxuriantly in a niche of low
temperature (LT) at high altitudes in Himalayan belt. The
plant is expected to harbor novel genes particularly for tolerance
to LT. Using differential display, two cDNAs
RaMPK1 and RaMPK2, showing homology to mitogenactivated
protein kinases (MAPKs) were isolated. As compared
to RaMPK1, RaMPK2 exhibited strong up-regulation
in response to LT. RaMPK1 was novel in terms of possessing
a small glutamine and proline rich region at the
N-terminal end. Secondly, though RaMPK1 showed
homology with salicylic acid (SA) responsive MAPKs, the
gene was down-regulated by SA but activated by jasmonate
(JA). Abscisic acid (ABA) and polyethylene glycol (PEG)
also down-regulated RaMPK1. RaMPK2 showed downregulation
within 5 min of exposure to JA and SA treatments,
followed by gradual increase in expression. Expression
of RaMPK2 was wavy in response to ABA and PEG
treatment. Results are discussed in light of the novelty of
these MAPKs.
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| Date |
2010
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/610/1/406_2010_9%2D12%2D2011.pdf
Ghawana, S and Kumar, Sanjay and Ahuja, Paramvir Singh (2010) Early low-temperature responsive mitogen activated protein kinases RaMPK1 and RaMPK2 from Rheum australe D. Don respond differentially to diverse stresses. Molecular Biology Reports, 37. pp. 933-938. |
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| Relation |
http://ihbt.csircentral.net/610/
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