Expression of recombinant Apple chlorotic leaf spot virus coat protein in heterologous system: production and use in immunodiagnosis
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Expression of recombinant Apple chlorotic leaf spot virus coat
protein in heterologous system: production
and use in immunodiagnosis
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| Creator |
Rana, T
Chandel, V Hallan, Vipin Zaidi, A A |
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| Subject |
Plant sciences
Plant viruses |
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| Description |
Expression condition for maximum recovery of
recombinant Apple chlorotic leaf spot virus (ACLSV) coat
protein was standardized. The in vitro expressed fusion
protein with 6xHis tag (~43 Kd) was purified from
inclusion bodies and used as an antigen for raising
polyclonal antiserum in rabbit. This antiserum consistently
detected ACLSV in pome and stone fruits as well as
herbaceous host plants by direct double antibody sandwich
enzyme linked immunosorbent assay (DAS-ELISA)
and direct tissue blot immunoassay (DTBIA). The
conditions for immuno-capture RT-PCR (IC-RT-PCR)
were also standardized.
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| Date |
2011
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/615/1/549_2011_9%2D12%2D2011.pdf
Rana, T and Chandel, V and Hallan, Vipin and Zaidi, A A (2011) Expression of recombinant Apple chlorotic leaf spot virus coat protein in heterologous system: production and use in immunodiagnosis. Journal of Plant Biochemistry and Biotechnology, 20. pp. 138-141. |
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| Relation |
http://ihbt.csircentral.net/615/
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