An efficient method of propagation of Podophyllum hexandrum: an endangered medicinal plant of the Western Himalayas under ex situ conditions
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
An efficient method of propagation of Podophyllum hexandrum:
an endangered medicinal plant of the Western Himalayas
under ex situ conditions
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| Creator |
Kharkwal, A C
Kushwaha , R Prakash , Om Ogra, R K Bhattacharya, Amita |
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| Subject |
Nutraceuticals
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| Description |
This study shows an effective but simple
method of conserving characterized populations and elite
clones through vegetative propagation and genetic diversity
through seeds in Podophyllum hexandrum (family
Berberidaceae). Seed dormancy has been considered to be
a major constraint in these seeds and most of the earlier
reports recommended dormancy-breaking pretreatments
such as chilling, gibberellic acid (GA3), etc. However,
seeds of the 14 accessions that we tested exhibited no
dormancy and hence did not require any pretreatments.
Besides accession, collection of seeds with high moisture
content could be one of the reasons for lack of dormancy.
Thus, we propose germination of seeds (while they still
retained moisture) in sand at 25 degree C for high and reproducible
results within a shorter period of time compared with
earlier reports. Hypocotyl dormancy is known to considerably
delay plant establishment and hence en masse
propagation by preventing the emergence of functional
leaves for up to 11–12 months. Manual removal of cotyledonary
leaves, being labor and time intensive, is not a
feasible method for large-scale seedling establishment.
However, in this study, we showed that GA3 at 200 ppm
can alleviate hypocotyl dormancy besides reducing the
time taken for true or functional leaf emergence. Treatment
of cotyledonary leaves of 1 week-old-seedlings with
200 ppm GA3 resulted in true or functional leaf emergence
within 7 days, and the resultant plants were also more
vigorous than the ones obtained from manual removal of
cotyledonary leaves. The study helped advance the establishment
of seedlings by one growing season (almost 1
year).
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| Date |
2008
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/299/1/273_2008_An_efficient.pdf
Kharkwal, A C and Kushwaha , R and Prakash , Om and Ogra, R K and Bhattacharya, Amita (2008) An efficient method of propagation of Podophyllum hexandrum: an endangered medicinal plant of the Western Himalayas under ex situ conditions. Journal of Natural Medicines, 62 (2). pp. 211-216. |
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| Relation |
http://ihbt.csircentral.net/299/
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