An efficient liquid culture system for tea shoot proliferation
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
An efficient liquid culture system for tea shoot proliferation
|
|
| Creator |
Pandey, S
Nagar , P K |
|
| Subject |
Plant Biotechnology
|
|
| Description |
The efficiency of thidiazuron in promoting tea shoot proliferation in liquid medium was evaluated. As compared to
6-benzyl adenine which induced hyperhydricity in the proliferated shoots in liquid medium, a progressive increase
in the multiplication rate together with healthy shoot growth was achieved when thidiazuron (2.5 to 5.0 μM) was
used instead of 6-benzyl adenine. Of the different liquid volumes compared in 250 ml Erlenmyer flasks, 20 ml
was the most effective. While an increase in the multiplication rate coupled with normal but healthy shoots was
observed under static and agitated conditions at this volume of liquid medium, hyperhydricity was induced in 50
ml liquid medium. Therefore, 20 ml static liquid medium with subculture periods at an interval six to eight weeks
seems to be a cost and labour effective process as compared to the existing protocols involving solid media with
subculture periods at 4 weeks interval.
|
|
| Date |
2002
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://ihbt.csircentral.net/358/1/31_2001_An_efficent.pdf
Pandey, S and Nagar , P K (2002) An efficient liquid culture system for tea shoot proliferation. Biol.Plant, 45. pp. 291-4. |
|
| Relation |
http://ihbt.csircentral.net/358/
|
|