Interactions of Berberine with Poly(A) and tRNA
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Interactions of Berberine with Poly(A) and tRNA
|
|
| Creator |
Nandi, R
Debnath, D Maiti, Motilal |
|
| Subject |
Chemistry
|
|
| Description |
The interaction of berberine chloride with poly(A) and tRNA has been studied by various spectroscopic techniques.
Binding parameters determined from spectrophotometric and spectrofluorimetric measurements by Scatchard analysis
indicate a very high effective binding capacity of berberine to poly(A) as compared to DNA or tRNA. The circular
dichroism studies show that binding of berberine to poly(A) causes a significant change in the circular dichroic spectrum
of poly(A) itself, as manifested by (i) a decrease of both positive and negative bands and (ii) appearance of a
conservative type of extrinsic circular dichroic spectrum in the wavelength range of 300-400 nm, while it does not cause
any significant alteration to the A form structure of tRNA. It is concluded that berberine interacts stronger with poly
(A) than DNA or tRNA. The results are interpreted in terms of its reported biological activities.
|
|
| Publisher |
Elsevier
|
|
| Date |
1990
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://www.eprints.iicb.res.in/1218/1/BIOCHIMICA_ET_BIOPHYSICA_ACTA__1049__(3_)339%2D342;1990[26].pdf
Nandi, R and Debnath, D and Maiti, Motilal (1990) Interactions of Berberine with Poly(A) and tRNA. BBA - Biochimica et Biophysica Acta, 1049 (3). pp. 339-342. |
|
| Relation |
http://dx.doi.org/10.1016/0167-4781(90)90107-D
http://www.eprints.iicb.res.in/1218/ |
|