Selective Th1 up-regulating activity of Withania somnifera aqueous extract in an experimental system using flow cytometry
IR@IIIM: CSIR-Indian Institute of Integrative Medicine, Jammu
View Archive Info| Field | Value | |
| Title |
Selective Th1 up-regulating activity of Withania somnifera aqueous extract in an experimental system using flow cytometry
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| Creator |
Bani, Sarang
Gautam, Manish Sheikh, Fayaz Ahmad Khan, Beenish Satti, Narender Kumar Suri, Krishan Avatar Qazi, Ghulam Nabi Patwardhan, Bhushan |
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| Subject |
Chemical Sciences
Pharmacological Sciences |
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| Description |
Withania somnifera (Ashwagandha) is reported to be immunoprotective and immunoadjuvant. We studied its roots aqueous extract on T helper(Th) immunity using flowcytometry. This extractwas standardized with six withanolides as marker compounds using HPLC. Once daily dose ranging from 25 to 400 mg/kg/p.o. was used to study effect on Th1: IFN-gamma, IL-2 and Th2: IL-4 cytokine modulation. We also studied effect on CD4 and CD8 in normal and immunesuppressed mice. The results indicate that extract at 100 mg/kg resulted significant selective up-regulation of Th1 response.Treatment with extract showed significant increase inCD4andCD8counts as compared to control and cyclopsorin A, with a faster recovery
of CD4+ T cells in immunesuppressed animals. Under immunesuppressed conditions, potentiation of cellular and humoral immune responses of extract was comparable to levamisole. This study indicates the selective Th1 up-regulating effect of extract and suggests its use for selective Th1/Th2 modulation.
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| Date |
2006
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://iiim.csircentral.net/337/1/10.1016j.jep.2006.02.016.pdf
Bani, Sarang and Gautam, Manish and Sheikh, Fayaz Ahmad and Khan, Beenish and Satti, Narender Kumar and Suri, Krishan Avatar and Qazi, Ghulam Nabi and Patwardhan, Bhushan (2006) Selective Th1 up-regulating activity of Withania somnifera aqueous extract in an experimental system using flow cytometry. Journal of Ethnopharmacology, 107 (1). pp. 107-115. ISSN 03788741 |
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| Relation |
http://dx.doi.org/10.1016/j.jep.2006.02.016
http://iiim.csircentral.net/337/ |
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