Molecular cloning of enantioselective ester hydrolase fromBacillus pumilusDBRL-191
IR@IIIM: CSIR-Indian Institute of Integrative Medicine, Jammu
View Archive Info| Field | Value | |
| Title |
Molecular cloning of enantioselective ester hydrolase fromBacillus pumilusDBRL-191
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| Creator |
Rasool, Shafaq
Johri, Sarojini Riyaz-ul-Hassan, Syed Maqbool, Qurrat-ul-Ain Verma, Vijeshwar Koul, Surrinder Taneja, Subhash C. Qazi, Ghulam N. |
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| Subject |
Biological Sciences
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| Description |
A gene from Bacillus pumilus expressed under its native promoter was cloned in Escherichia coli. Recombinant B. pumilus esterase(BPE) affects the kinetic resolution of racemic mixtures such as unsubstituted and substituted 1-(phenyl)ethanols (E � 33–103), ethyl 3-hydroxy-3-phenylpropanoate (E � 45–71), trans-4-fluorophenyl-3-hydroxymethyl-N-methylpiperidine (E � 10–13) and ethyl 2-
hydroxy-4-phenylbutyrate (E � 7). The enzyme is composed of a 34-amino acid signal peptide and a 181-amino acid mature protein corresponding to a molecular weight of �19.2 kD and pI � 9.4. 3-D the structural model of the enzyme built by homology modelling using the atomic coordinates from the crystal structure of B. subtilis lipase (LipA) showed a compact minimal a/b hydrolase fold.
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| Date |
2005
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://iiim.csircentral.net/296/1/10.1016j.femsle.2005.06.022.pdf
Rasool, Shafaq and Johri, Sarojini and Riyaz-ul-Hassan, Syed and Maqbool, Qurrat-ul-Ain and Verma, Vijeshwar and Koul, Surrinder and Taneja, Subhash C. and Qazi, Ghulam N. (2005) Molecular cloning of enantioselective ester hydrolase fromBacillus pumilusDBRL-191. FEMS Microbiology Letters, 249 (1). pp. 113-120. ISSN 03781097 |
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| Relation |
http://dx.doi.org/10.1016/j.femsle.2005.06.022
http://iiim.csircentral.net/296/ |
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