Separation, identification, and quantification of selected withanolides in plant extracts ofWithania somnifera by HPLC-UV(DAD) - Positive ion electrospray ionisation-mass spectrometry
IR@IIIM: CSIR-Indian Institute of Integrative Medicine, Jammu
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| Title |
Separation, identification, and quantification of selected withanolides in plant extracts ofWithania somnifera by HPLC-UV(DAD) - Positive ion electrospray ionisation-mass spectrometry
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| Creator |
Khajuria, Ravi K.
Suri, Krishan A. Gupta, Rajinder K. Satti, Naresh K. Amina, Musarat Suri, Om P. Qazi, Ghulam N. |
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| Subject |
Chemical Sciences
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| Description |
This paper describes a method for separation, identification, and quantification of selected withanolides in Withania somnifera plant extracts by HPLC-UV(DAD)–Mass
Spectrometry (HPLC-MS). Withaferin-A (WS-3), 12-deoxywithastramonolide (WS-12DS), Withanolide A (WS-1), and Withanone (WS-2) were used as external standards.
The compounds were isolated from Withania somnifera by repeated column chromatography of the root extract and their identity was established by 1H- and 13CNMR
and mass spectral data. The compounds were chromatographed on a Merck(25064.6 mm ID, 5 lm) column and analyzed by Electrospray Ionization on a mass spectrometer in Selected Ion Mode (SIM). For quantification, [M + Na]+ ions were
monitored. Linear calibration curves were obtained in the concentration range of 1.50 lg/mL to 6.5 lg/mL. The method was applied successfully to the detection and
quantification of the said withanolides in a number of samples.
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| Date |
2004
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://iiim.csircentral.net/340/1/10.1002jssc.200301690.pdf
Khajuria, Ravi K. and Suri, Krishan A. and Gupta, Rajinder K. and Satti, Naresh K. and Amina, Musarat and Suri, Om P. and Qazi, Ghulam N. (2004) Separation, identification, and quantification of selected withanolides in plant extracts ofWithania somnifera by HPLC-UV(DAD) - Positive ion electrospray ionisation-mass spectrometry. Journal of Separation Science, 27 (7-8). pp. 541-546. ISSN 1615-9306 |
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| Relation |
http://dx.doi.org/10.1002/jssc.200301690
http://iiim.csircentral.net/340/ |
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