Protective efficacy of mitochondrial targeted antioxidant MitoQ against dichlorvos induced oxidative stress and cell death in rat brain
IR@IIIM: CSIR-Indian Institute of Integrative Medicine, Jammu
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| Title |
Protective efficacy of mitochondrial targeted antioxidant MitoQ against dichlorvos induced oxidative stress and cell death in rat brain
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| Creator |
Wani, Willayat Yousuf
Gudup, Satish Sunkaria, Aditya Bal, Amanjit Singh, Parvinder Pal Kandimalla, Ramesh J.L. Sharma, Deep Raj Gill, Kiran Dip |
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| Subject |
Pharmacological Sciences
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| Description |
Dichlorvos is a synthetic insecticide that belongs to the family of chemically related organophosphate(OP) pesticides. It can be released into the environment as a major degradation product of other OPs, such as trichlorfon, naled, and metrifonate. Dichlorvos exerts its toxic effects in humans and animals by inhibiting neural acetylcholinesterase. Chronic low-level exposure to dichlorvos has been shown to result in inhibition of the mitochondrial complex I and cytochrome oxidase in rat brain, resulting in generation of reactive oxygen species (ROS). Enhanced ROS production leads to disruption of cellular antioxidant defense systems and release of cytochrome c (cyt c) from mitochondria to cytosol resulting in apoptotic cell death. MitoQ is an antioxidant, selectively targeted to mitochondria and protects it from oxidative damage and has been shown to decrease mitochondrial damage in various animal models of oxidative
stress. We hypothesized that if oxidative damage to mitochondria does play a significant role in dichlorvos induced neurodegeneration, then MitoQ should ameliorate neuronal apoptosis. Administration of MitoQ (100 mmol/kg body wt/day) reduced dichlorvos (6 mg/kg body wt/day) induced oxidative stress (decreased ROS production, increased MnSOD activity and glutathione levels) with decreased lipid peroxidation, protein and DNA oxidation. In addition, MitoQ also suppressed DNA fragmentation, cyt c
release and caspase-3 activity in dichlorvos treated rats compared to the control group. Further electron
microscopic studies revealed that MitoQ attenuates dichlorvos induced mitochondrial swelling, loss of
cristae and chromatin condensation. These results indicate that MitoQ may be beneficial against OP (dichlorvos) induced neurodegeneration.
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| Date |
2011
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://iiim.csircentral.net/318/1/10.1016j.neuropharm.2011.07.008.pdf
Wani, Willayat Yousuf and Gudup, Satish and Sunkaria, Aditya and Bal, Amanjit and Singh, Parvinder Pal and Kandimalla, Ramesh J.L. and Sharma, Deep Raj and Gill, Kiran Dip (2011) Protective efficacy of mitochondrial targeted antioxidant MitoQ against dichlorvos induced oxidative stress and cell death in rat brain. Neuropharmacology, 61 (8). pp. 1193-1201. ISSN 00283908 |
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| Relation |
http://dx.doi.org/10.1016/j.neuropharm.2011.07.008
http://iiim.csircentral.net/318/ |
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