Binding of Estradiol-Peroxidase Conjugate to Estrogen Receptor
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Binding of Estradiol-Peroxidase Conjugate to
Estrogen Receptor
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| Creator |
Bhattacharjee, Maitreyi
Ali, Esahak |
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| Subject |
Drug Development/Diagnostics & Biotechnology
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| Description |
The binding of estradiol-horseradish peroxidase conjugate to rat uterine cytosolic
estrogen receptor was studied. The conjugate having a steroid to enzyme ratio of 2.8 : 1 was
allowed to bind to protamine precipitated receptor in presence or absence of 100-fold excess
of free estradiol. The bound enzyme activity was measured and the data subjected to Scatchard
analysis to obtain the dissociation constant and the number of binding sites. Although the
binding parameter so obtained differed from values obtained using radiolabelled estradiol, the
method may be used for comparative studies.
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| Publisher |
Elsevier
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| Date |
1990
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1224/1/JOURNAL_OF_STEROID_BIOCHEMISTRY_AND_MOLECULAR_BIOLOGY__37(_5)_693%2D696;1990[1].pdf
Bhattacharjee, Maitreyi and Ali, Esahak (1990) Binding of Estradiol-Peroxidase Conjugate to Estrogen Receptor. The Journal of Steroid Biochemistry and Molecular Biology, 37 (5). pp. 693-696. |
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| Relation |
http://dx.doi.org/10.1016/0960-0760(90)90353-M
http://www.eprints.iicb.res.in/1224/ |
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