Segregational and structural instability of recombinant plasmid carrying genes for naphthalene degrading pathway.
IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh
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| Title |
Segregational and structural instability of recombinant plasmid carrying genes for naphthalene degrading pathway.
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| Creator |
Samanta, S K
Rani, M Jain, R K |
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| Subject |
QR Microbiology
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| Description |
The stability of recombinant plasmid carrying genes for naphthalene mineralization was determined. A strain of Pseudomonas putida capable of mineralizing naphthalene (Nap+) via salicylate (Sal+) was isolated, and all regulatory and structural genes for the whole pathway were found to be encoded on a 25 kb EcoRI fragment of an approximately 83 kb plasmid present in this strain. The 25 kb EcoRI fragment was cloned into a tetracycline-resistant (TcR) cloning vector pLAFR3 and the recombinant plasmid, pRKJ3 (Nap+, Sal+, TcR), thus obtained was transferred into the plasmid-free strain Pseudomonas putida KT2442 in order to test the stability of the plasmid. Plasmid pRKJ3 was found to be segregationally and/or structurally unstable, depending on the growth conditions. Two types of novel derivative strains having the phenotypes Nap-, Sal+, TcR and Nap-, Sal-, TcR with specific deletions of approximately 2 kb and 18 kb, respectively, were obtained.
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| Publisher |
Wiley
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| Date |
1998-04
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://crdd.osdd.net/open/353/1/jain98.pdf
Samanta, S K and Rani, M and Jain, R K (1998) Segregational and structural instability of recombinant plasmid carrying genes for naphthalene degrading pathway. Letters in applied microbiology, 26 (4). pp. 265-9. ISSN 0266-8254 |
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| Relation |
http://onlinelibrary.wiley.com/doi/10.1046/j.1472-765X.1998.00316.x/pdf
http://crdd.osdd.net/open/353/ |
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