Biotransformation of tea catechins into theaflavins with immobilized polyphenol oxidase
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
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| Title |
Biotransformation of tea catechins into theaflavins with immobilized
polyphenol oxidase
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| Creator |
Sharma, K
Bari, S S Singh , H P |
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| Subject |
Synthetic Chemistry
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| Description |
Theaflavins, an active antioxidant, a natural pigment and pharmacologically active molecule obtained from
black tea were bioprocessed on an immobilized tea polyphenol oxidase (PPO) system by the conversion
of tea catechins extracted from green tea leaves with an overall conversion efficiency of 85% about 14-fold
increase over maximum achievable in normal black teas. The immobilized enzyme (IE) system consists
of activated cellulose matrix on to which the freshly extracted tea leaf polyphenol oxidase was covalently
linked. Cellulose as a matrix of choice was considered primarily for its non-toxic nature, natural origin,
low cost and easy availability. The kinetic parameters of the IE system were; protein loading capacity
11.8 mg/g; pH optimum 5.9; temperature optimum 37.5 ◦C; Km 4.76±0.08mM; Vmax 20±1.80 nmol/min;
enzyme activity retention (83.58%) and number of batches per turnover without loss of activity was 14.
The product from IE system was identified by HPLC and ESI-QTOF-MS spectrometry.
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| Date |
2009
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/891/1/hps%2Dkapil.pdf
Sharma, K and Bari, S S and Singh , H P (2009) Biotransformation of tea catechins into theaflavins with immobilized polyphenol oxidase. Journal of Molecular Catalysis B:Enzymatic, 56 (4). pp. 253-258. |
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| Relation |
http://ihbt.csircentral.net/891/
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