Comparative analysis of protein unfoldedness in human housekeeping and non-housekeeping proteins
IR@IGIB: CSIR-Institute of Genomics & Integrative Biology, New Delhi
View Archive Info| Field | Value | |
| Title |
Comparative analysis of protein unfoldedness
in human housekeeping and non-housekeeping
proteins
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| Creator |
Pandey, Neeraj
Ganapathi, Mythily Kumar, Kaushal Dasgupta, Dipayan Sutar, Sushanta Kumar Das Dash, Debasis |
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| Subject |
BI1 Bioinformatics (General)
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| Description |
Absence of any regular structure is increasingly
being observed in structural studies of proteins. These dis-
ordered regions or random coils, which have been observed
under physiological conditions, are indicators of protein plas-
ticity. The wide variety of interactions possible due to the
flexibility of these ‘natively disordered’ regions confers func-
tional advantage to the protein and the organism in general.
This concept is underscored by the increasing proportion of
intrinsically unstructured proteins seen with the ascension in
the complexity of the organisms.
The ‘natively unfolded/disordered’ state of the protein can be
predicted utilizing Uversky’s or Dunker’s algorithm. We utilized
Uversky’s prediction scheme and based on the unique position
of a protein in the charge–hydrophobicity plot, a derived net
score was used to predict the overall disorder of the human
housekeeping and non-housekeeping proteins. Substantial
numbers of proteins in both the classes were predicted to
be unfolded. However, comparative genomic analysis of pre-
dicted unfolded Homo sapiens proteins with homologues in
Caenorhabditis elegans, Drosophila melanogaster and Mus
musculus revealed significant increase in unfoldedness in
non-housekeeping proteins in comparison with housekeep-
ing proteins. Our analysis in the evolutionary context sug-
gests addition or substitution of amino acid residues which
favour unfoldedness in non-housekeeping proteins compared
to housekeeping proteins.
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| Date |
2004-06-04
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://openaccess.igib.res.in/115/1/Hkp%2DnonHkp.pdf
Pandey, Neeraj and Ganapathi, Mythily and Kumar, Kaushal and Dasgupta, Dipayan and Sutar, Sushanta Kumar Das and Dash, Debasis (2004) Comparative analysis of protein unfoldedness in human housekeeping and non-housekeeping proteins. Comparative analysis of protein unfoldedness in human housekeeping and non-housekeeping proteins, 20 (17). pp. 2904-2910. |
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| Relation |
http://openaccess.igib.res.in/115/
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