Nonenzymatic Reduction of Hydrogen Peroxide Produced during the Bioelectrocatalysis of Glucose Oxidase on Urchin-like Nanofibrillar Structures of Cu on Au Substrates
IR@CECRI: CSIR-Central Electrochemical Research Institute, Karaikudi
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| Title |
Nonenzymatic Reduction of Hydrogen Peroxide Produced during the Bioelectrocatalysis of Glucose Oxidase on Urchin-like Nanofibrillar Structures of Cu on Au Substrates
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| Creator |
Vijayalakshmi, A.
Karthikeyan, R. Sheela, B. |
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| Subject |
Electrodics and Electrocatalysis
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| Description |
This work describes the nonenzymatic reduction of hydrogen peroxide on urchin-like nanofibrillar structures
of Cu formed on gold substrates modified by a self-assembled monolayer (SAM) of p-mercaptobenzoic acid.
Because the detection is based on the electrochemical reduction, the method is less prone to interference
from easily oxidizable biologically important compounds such as dopamine, uric acid, and ascorbic acid.
This method is extended to the detection of glucose by immobilizing glucose oxidase on the nanocomposite
electrode with the help of chitosan. The linear detection range for glucose is 100 nM to 1800 nM. Sensitivity
of detection is 1.99 nA/nM. The Michaelis-Menten constant is very low (125 nM), indicating high binding
affinity of the enzyme to the substrate.
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| Publisher |
American Chemical Society
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| Date |
2010
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://cecri.csircentral.net/470/1/2010-096.pdf
Vijayalakshmi, A. and Karthikeyan, R. and Sheela, B. (2010) Nonenzymatic Reduction of Hydrogen Peroxide Produced during the Bioelectrocatalysis of Glucose Oxidase on Urchin-like Nanofibrillar Structures of Cu on Au Substrates. Journal of Physics and Chemistry, 114. pp. 22159-22164. |
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| Relation |
http://cecri.csircentral.net/470/
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