UDP-Glucose Dehydrogenase from Capra Hircus Liver: Purification, Partial Characterization and Evaluation as a Coupling Enzyme in UDP-Galactose 4-Epimerase Assay
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
UDP-Glucose Dehydrogenase from Capra Hircus Liver: Purification, Partial
Characterization and Evaluation as a Coupling Enzyme in UDP-Galactose 4-Epimerase Assay
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| Creator |
Banerjee, Nupur
Bhattacharyya, Debasish |
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| Subject |
Structural Biology & Bioinformatics
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| Description |
UDP-glucose dehydrogenase from Capra hircus has been purified to homogeneity by salt fractionations,
heat treatment and chromatographic steps. It is a homohexamer of about 300 kDa. Though the basic physical
and enzymatic properties of the caprine enzyme are comparable to those of the beef liver enzyme, it
has lower energy of activation and different entropy and enthalpy for the transition state during catalysis.
The caprine enzyme can act suitably as an auxiliary enzyme in the coupled assay system for UDP-galactose
4-epimerase
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| Date |
2011
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1296/1/JOURNAL_OF_MOLECULAR_CATALYSIS_B%2DENZYMATIC__68(_1)_37%2D43_;2011[155].pdf
Banerjee, Nupur and Bhattacharyya, Debasish (2011) UDP-Glucose Dehydrogenase from Capra Hircus Liver: Purification, Partial Characterization and Evaluation as a Coupling Enzyme in UDP-Galactose 4-Epimerase Assay. Journal of Molecular Catalysis B: Enzymatic, 68 (1). pp. 37-43. |
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| Relation |
http://dx.doi.org/10.1016/j.molcatb.2010.09.007
http://www.eprints.iicb.res.in/1296/ |
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